Research Interests

The Team





Most Recent Publications:

  1. Wong A, Lange D, Houle S, Arbatsky, Valvano MA, Knirel KA, Dozois C and Creuzenet C. Role of capsular modified heptose in the virulence of Campylobacter jejuni. Molecular Microbiology. In press.

  2. Lester J., Kichler S., Oickle B., Fairweather S., Oberc A., Chahal J., Ratnayake D. and Creuzenet C. (2015) Characterization of Helicobacter pylori HP0231 (DsbK): role in disulfide bond formation, redox homeostasis and production of Helicobacter cystein-rich protein HcpE. Molecular Microbiology. In press 

  3. Lee MJ, Gravelat FN, Cerone RP, Baptista S, Campoli P., Choe SI, Kravtsov I, Vinogradov E, Creuzenet C, Liu H, Berghuis A, Latge JP, Filler SG, Fontaine T, Sheppard DC. (2014) Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides. J. Biol. Chem 289, 1243-1256.

  4.  McCallum M., Shaw GS. and Creuzenet C. (2013) Comparison of predicted epimerases and reductases of the Campylobacter jejuni D-altro- and L-gluco- heptose synthesis pathways. J. Biol. Chem. 288, 19569–19580.
  5. Bui XT, Qvortrup K, Wolff A, Bang DD, and Creuzenet C (2012)Effect of environmental stress factors on the uptake and survival of Campylobacter jejuni in Acanthamoeba castellanii. BMC Microbiology 12:232.
  6. McCallum M, Shaw SD, Shaw GS, and Creuzenet C (2012). Complete 6-deoxy-D-altro-heptose biosynthesis pathway from Campylobacter jejuni: MORE COMPLEX THAN ANTICIPATED. J Biol Chem. 287(35):29776-29788.
  7. Bui XT, Winding A, Qvortrup K, Wolff A, Bang DD, and Creuzenet C (2012). Survival of Campylobacter jejuni in co-culture with Acanthamoeba castellanii: Role of amoeba-mediated depletion of dissolved oxygen. Environmental Microbiology 14(8):2034-2047.
  8. McCallum M, Shaw GS, and Creuzenet C (2011). Characterization of the dehydratase WcbK and the reductase WcaG involved in GDP-6-deoxy-manno-heptose biosynthesis in Campylobacter jejuni. J Biol Chem. 439(2):235-248.
  9. Hopf PS, Ford RS, Zebian N, Merkx-Jacques A, Vijayakumar S, Ratnayake D, and Creuzenet C (2011). Protein glycosylation in Helicobacter pylori: Beyond the flagellins? PloS One 6(9):e25722.
  10. Butty FD, Aucoin M, Morrison L, Ho N, Shaw GS, and Creuzenet C (2009). Elucidating the formation of 6-deoxyheptose: Biochemical characterization of the GDP-D-glycero-d-manno-heptose C6 dehydratase, DmhA, and its associated C4 reductase, DmhB. Biochemistry 48(32):7764-7775.
  11. Demendi M, and Creuzenet C (2009). Cj1123c (PgID), a multifaceted acetyltransferase from Campylobacter jejuni. Biochemistry and Cell Biology 87(3):469-483.
  12. Kondakova AN, Ho N, Bystrova OV, Shashkov AS, Lindner B, Creuzenet C and Knirel YA (2008). Strutural studies of the O-antigens of Yersinia pseudotuberculosis O:2a and mutants thereof with impaired 6-deoxy-D-manno-heptose biosynthesis pathway. Carbohydrate Research 343(8):1383-1389.
  13. Ho N, Kondakova AN, Knirel YA, and Creuzenet C (2008). The biosynthesis and biological role of 6-deoxyheptoses in the lipopolysaccharide O-antigen of Yersinia pseudotuberculosis. Molecular Microbiology 68(2):424-447.
  14. Vijayakumar S*, Merkx-Jacques A*, Ratnayake DB, Gryski I, Obhi RK, Houle S, Dozois C and Creuzenet C (2006). Cj1121c, a novel UDP-4-keto-6-deoxy-GlcNAc C4 aminotransferase essential for protein glycosylation and virulence in Campylobacter jejuni. J Biol Chem. 281:27733-27743. Equal contribution of these two authors.
  15. Ishiyama N, Creuzenet C, Miller WL, Demendi M, Anderson EM, Harauz G, Lam JS, Berghuis AM. (2006) Structural studies of FlaA1 from Helicobacter pylori reveal the mechanism for inverting 4,6-dehydratase activity. J Biol Chem. 281:24489-24495.
  16. Obhi RK and Creuzenet C (2005). Biochemical characterization of the Campylobacter jejuni Cj1294, a novel UDP-4-keto-6-deoxy-GlcNAc aminotransferase that generates UDP-4-amino-4,6-dideoxy-GalNAc. J Biol Chem. 280:20902-20908.
  17. Demendi M, Ishiyama N, Lam JS, Berghuis AM, Creuzenet C (2005). Towards a better understanding of the substrate specificity of the UDP-N-acetylglucosamine C4 epimerase WbpP.Biochem J. 389:173-180.
  18. Bourke B, Ceponis P, Chiba N, Czinn S, Ferraro R, Fischbach L, Gold B, Hyunh H, Jacobson K, Jones NL, Koletzko S, Lebel S, Moayyedi P, Ridell R, Sherman P, van Zanten S, Beck I, Best L, Boland M, Bursey F, Chaun H, Cooper G, Craig B, Creuzenet C, Critch J, Govender K, Hassall E, Kaplan A, Keelan M, Noad G, Robertson M, Smith L, Stein M, Taylor D, Walters T, Persaud R, Whitaker S, Woodland R; Canadian Helicobacter Study Group (2005). Canadian Helicobacter study group consensus conference: update on the approach to Helicobacter pylori infection in children and adolescents - an evidence-based evaluation. J. Gastroenterology 19:399-408.
  19. Ishiyama N, Creuzenet C, Lam JS, Berghuis AM (2004). Crystal structure of WbpP, a genuine UDP-N-acetylglucosamine 4-epimerase from Pseudomonas aeruginosa: Substrate specificity in UDP-Hexose 4-epimerases. J. Chem. 279(21):22635-42.
  20. Steiner-Mosonyi M, Creuzenet C, Keates RA, Strub BR, Mangroo D (2004). The Pseudomonas aeruginosa initiation factor IF-2 is responsible for formylation-independent protein initiation in P. aeruginosa. J Biol Chem. 279(50):52262-9.
  21.  Merkx-Jacques A, Obhi RK, Bethune G, Creuzenet C (2004). The Helicobacter pylori flaA1 and wbpB genes control lipopolysaccharide and flagella synthesis/function. J bacteriol. 186(8):2253-65.
  22. Creuzenet C (2004). Characterization of CJ1293, a new UDP-GlcNAc C6-dehydratases from Campylobacter jejuni. FEBS Letters. 559:136-140.
  23.  Popot JL, Berry EA, Charvolin D, Creuzenet C et al.. (2003). Amphipols: polymeric surfactants for membrane biology research. Cell Mol Life Sci. 60(8):1559-74.
  24. Creuzenet C, Urbanic R and Lam JS (2002). Structure function studies of two novel UDP-GlcNAc C6-dehydratases / C4 reductases: variation from the SYK dogma. J Biol Chem. 277:26769-26778.
  25. Creuzenet C. and Lam JS (2001). Topological and functional characterisation of WbpM, an inner-membrane UDP-GlcNAc C6 dehydratase essential for lipopolysaccharide biosynthesis in Pseudomonas aeruginosa. Molecular Microbiology 41(6):1295-310.
  26. Creuzenet C, Schurr M, Wakarchuk W, Li J and Lam JS (2000). FlaA1, a new bifunctional UDP-GlcNAc C6 dehydratase / C4 reductase from Helicobacter pylori. J. Biol. Chem. 275: 34873-34880.
  27. Zhao X, Creuzenet C, Belanger M, Egbosimba E, Li J and Lam JS (2000). WbpO, a UDP-N-acetyl D-galactosamine dehydrogenase from Pseudomonas aeruginosa serotype O6. J. Biol. Chem. 275: 33252-33259.
  28. Blankenfeldt W, Giraud MF, Leonard G, Rahim R, Creuzenet C, Lam JS and Naismith JH (2000). The purification, crystallisation and preliminary structural characterization of glucose-1-phosphate thymidyltransferase (RmlA), the first enzyme of the dTDP-L-rhamnose synthesis pathway from Pseudomonas aeruginosa. Acta Cryst. D56: 1501-1504.
  29. Creuzenet C, Belanger M, Wakarchuk W and Lam JS (2000). Expression, purification and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6. J. Biol. Chem. 275: 19060-19067.
  30. Newton T, Creuzenet C and Mangroo D (1999). Formylation is not essential for initiation of protein synthesis in all eubacteria. J. Biol. Chem. 274: 22143-22146.
  31. Creuzenet C and Mangroo D (1998). Physico-chemical characterization of human Von Ebner's gland protein expressed in E. coli. Implications for its physiological role. Protein Expr. Purif. 14:254-260..
  32. Thurmond RL*, Creuzenet C*, Reeves PJ and Khorana G (1997). Structure and function in rhodopsin. Peptide sequences in the cytoplasmic loops of rhodopsin are intimately involved in interaction with rhodopsin kinase. Proceedings of the National Academy of Science (USA) 94: 1715-1720. * Equal contribution of these two authors.
  33. Creuzenet C, Durand C and Haertlé T (1997). Interactions of alphas2- and beta-casein signal peptides with DMPC and DMPG liposomes. Peptides. 18: 463-472.

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Last updated: March 24, 2015