Transposons are integral components of genomes in essentially all organisms.
While their mobilization can be destructive to their hosts, transposons can
contribute to host fitness by providing a reservoir of DNA sequences that their
hosts can use. This includes regulatory DNA sequences for controlling host
transcription and protein coding sequences for the production of specialized DNA
cleavage/ligation proteins. We are working towards establishing a new paradigm
for transposon ‘domestication’ wherein a transposon contributes regulatory RNAs
to control host gene expression at the post-transcriptional level. In the course
of studying regulatory mechanisms controlling transposition of a bacterial
insertion sequence in E. coli and Salmonella, we identified transposon-encoded
RNAs that have the capacity to regulate host gene expression. Notably this
includes genes that are important for pathogenicity and carbohydrate metabolism.
Current projects in the lab focus on:
1. Defining targets of transposon-encoded regulatory RNAs in a variety of
bacterial species
2. Defining mechanisms through which transposon-encoded regulatory RNAs regulate
host gene expression
3. Determining if transposon-encoded regulatory RNAs play and important role in
pathogenesis and metabolism
4. Determining if transposon-encoded regulatory RNAs co-evolve with their
targets