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Some information about me

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Name: John Alan Kiernan. Born: 1942; Kidderminster, Worcestershire, England. Married: 1967 to Tessa Mary Westcott; 5 children; 10 grandchildren. Job(s): Recently retired from teaching neuroanatomy to medical and other students; research in neuroscience and in developing and improving histological and histochemical techniques. Occasional consultant for medical devices companies, patent law firms, publishers and universities, mostly in USA, also Asia.  Secretary and a trustee  of the Biological Stain Commission. Resides in:  Port Stanley, Ontario, Canada.  (This is in the middle of the North Shore of Lake Erie, 45 km south of London, and about equidistant from Toronto, Buffalo and Detroit, all about a 150 minutes drive.)   Other interests: Field botany (vascular plants).
Present professional address:   Dr J. A. Kiernan, Professor Emeritus, Department of Anatomy and Cell Biology, The University of Western Ontario, London, Canada N6A 5C1. Email:   kiernan[AT]uwo.ca   (Edit to change [AT] to @ if you paste this address to an emailing program.)	Link to web site for the Department of Anatomy & Cell Biology at UWO Link to the web site of the Biological Stain Commission

Education:

1953-1960:	King Edward VI Grammar School, Five Ways, Birmingham, England.
1960-1966 and 1967-1969:	University of Birmingham, England.

Degrees etc.

1963:	BSc (Anatomical Studies) University of Birmingham, England.
1966:	MB, ChB University of Birmingham, England.
1966:	MRCS England; LRCP London.
1969:	PhD (Faculty of Medicine) University of Birmingham, England.
1969:	MA (conferred by statute as a condition of appointment) University of Cambridge, England.
1979:	DSc (by thesis, through Department of Anatomy) University of Birmingham, England.

Employment:

1966:   House Surgeon, Accident Unit, East Birmingham Hospital, Birmingham, England.

1967: House Physician, Worcester Royal Infirmary, Worcester, England.

1967-1969:  Research Fellow (MRC), Department of Anatomy, University of Birmingham, England.

Membership of societies, editorial boards etc.

• 1957-    : Botanical Society of the British Isles (Member)
• 1958-    : Birmingham Natural History Society (Member)
• 1967-    : Principal List (Licence to Practise), General Medical Council (U.K.)
• 1968-    : Anatomical Society of Great Britain and Ireland (Member; Senior Member 2008-  )
• 1969-2009: Cambridge Philosophical Society (Fellow)
• 1974-1981:   Canadian Association of Anatomists (Member)
• 1976-    : The Histochemical Society (Member)
• 1980-1987:   Society for Neuroscience (Member)
• 1982-    : Southern Ontario Neuroscience Association (Member)
• 1985-2009: British Neuroscience Association (formerly Brain Research Association) (Member)
• 1985-2009: Royal Microscopical Society (Fellow)
• 1987-    : Canadian Association for Neuroscience (Member)
• 1993-    : Biological Stain Commission (Member; also a Trustee since 1995; Secretary and webmaster, 2008-    )
• 1995-    : Field Botanists of Ontario (Member)
• 1998-     : Society for Histotechnology (Member)
• 1995-    : Editorial Board of Biotechnic and Histochemistry
• 1998-2000 and 2006-    :  Editorial Board of The Journal of Histotechnology
• 2006-     : Associate Editor, The Journal of Histotechnology

Undergraduate teaching (University of  Birmingham)

• Medical students, 3rd year. Neuroanatomy Demonstrator (in an integrated Neurology course; 3 hr per week. 1963-1965 and 1967-1969).

Undergraduate teaching (University of Cambridge)

• Medical students, 2nd year. Lectures, laboratory and tutorial teaching in Anatomy, Histology, Neuroanatomy and Clinical Pharmacology. 6  hr per week. 1969-1972.

Undergraduate teaching (University of Western Ontario):

• Medical students, 1st/2nd year. Neuroanatomy (as part of an integrated Neurosciences course); 45 hours lectures and laboratory (1972-1997).
• Medical students, 4th (final) year. Clinical Neuroanatomy (Anatomy 9565); 18 hours laboratory demonstrations & tutorials; (1977-2011 ).
• Dental students, 1st year. Neuroanatomy; 16 hours lectures and laboratory; (1976-1985).
• Physiotherapy, Occupational Therapy and some others. Anatomy 250 (Neuroanatomy). 52 hours lectures & laboratory (1978-1997); also Anatomy 350a (Neuroanatomy): occasional contributions, mainly laboratory, about 15 hours (1997-2002); course manager and principal instructor, 42 hrs (2002-2006 ).

Graduate courses taught (University of Western Ontario):

• Anatomy 530a. Human Neuroanatomy. Occasional contributions, mainly laboratory; about 15 hours (1994-2001) ; course manager and principal instructor, 42 hrs (2002-2006 ).
• Anatomy 531a/b. Advanced Human Neuroscience (until 1997). About 20 hours, tutorial teaching.
• Anatomy 540. Research Methods in Anatomy (1997-2002). Coordinator, and 10 hours lectures, 4-12 hours laboratory.
• Anatomy 543a/b. Histochemistry (1976-2004). Coordinator and sole teacher, about 20 hours tutorial teaching and 10-15 hours laboratory.
• Anatomy 545y. Human Neuroanatomy (until 1994). Coordinator, and 16 hours lectures.
• Anatomy 535b. Anatomical Foundations of Neuroscience (1998- 2011). Coordinator and sole teacher. 36 hours lectures and laboratory Number changed to 9535b in 2008..
• Neuroscience 500. Principles of Neuroscience. (1996-2008). Systems section: Structural basis of nervous system circuitry; One lecture, 2 hours. 

  Link: Neuroanatomy at UWO

Research interests:

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• Axonal regeneration within and between different parts of the central and peripheral nervous systems.
• Trophic actions of motor nerves on skeletal muscle.
• Blood-tissue barriers to exogenous tracers and endogenous plasma proteins in different parts of the central and peripheral nervous systems.
• Mast cells and their involvement in axon reflex vasodilatation and neurogenic inflammation.
• Histopathology and pathogenesis of human motor neuron diseases, especially sporadic amyotrophic lateral sclerosis.
• Mechanisms of cell death due to injury and disease.
• Understanding, development and improvement of histological and histochemical techniques.

Publications

Peer reviewed papers Books Chapters Other publications

Items are colour-coded by subject:

• Black   for   Axonal regeneration, Neuropathology and Blood-tissue barriers.  (This includes papers concerned with interactions between vascular permeability and axonal regeneration.)
• Blue   for method-related   Histological and histochemical stuff. (This category includes papers in which my interest is in a technique, even though this may not be what the paper is mainly about.)
• Brown   for   Trophic interactions of neurons and other cells, especially muscle.
• Green   for   Botany.
• Magenta   for   Mast cells. (Most of these concern interactions of mast cells with neurons.)
• Red   for normal   Neuroanatomy and Neurohistology  (Most of these items are textbooks or other teaching publications.)
• This Orange color   is for   Other things that do not fit in any of the above categories. There are not many of these.

Papers in peer-reviewed journals

1. Holmes, R. L. and Kiernan, J. A. 1964. The fine structure of the infundibular process of the hedgehog. Zeitschrift fur Zellforschung 61, 894-912.
2. Kiernan, J. A. 1964. Carboxylic esterases of the hypothalamus and neurohypophysis of the hedgehog. Journal of the Royal Microscopical Society 83, 297-306.
3. Campbell, D. J. and Kiernan, J. A. 1966. Mast cells in the central nervous system. Nature 210, 756-757.
4. Kiernan, J. A. 1966. Notes on the flora of Worcestershire: additions to the flora since 1909. Proceedings of the Birmingham Natural History Society 20, 19-37.
5. Kiernan, J. A. 1967. On the probable absence of retino-hypothalamic connections in five mammals and an amphibian. Journal of Comparative Neurology 131, 405-408.
6. Kiernan, J. A. 1968. Effect of interrupting retino-hypothalamic connections on the melanophore response in Xenopus laevis. Experientia 24, 503-504.
7. Kiernan, J. A. 1968. Notes on the flora of Worcestershire. II. Further additions to the flora since 1909. Proceedings of the Birmingham Natural History Society 21, 83-88.
8. Kiernan, J. A. 1970. Silver staining of axons in subcellular fractions of nervous tissue. Experientia 26, 1352.
9. Kiernan, J. A. 1971. Pituicytes and the regenerative properties of neurosecretory and other axons in the rat. Journal of Anatomy 109, 97-114.
10. Kiernan, J. A. and Rawcliffe, P. M. 1971. Effects of triiodothyronine on the cerebellar cortex of the new-born rat in tissue culture. Experientia 27, 678-679.
11. Kiernan, J. A. and Pettit, D. R. 1971. Organ culture of the central nervous system of the adult rat. Experimental Neurology 32, 111-120.
12. Fertig, A., Kiernan, J. A. and Seyan, S. S. A. S. 1971. Enhancement of axonal regeneration in the brain of the rat by corticotrophin and triiodothyronine. Experimental Neurology 33, 372-385.
13. Kiernan, J. A. 1971. Rhynchosinapis - The Worcestershire records. Watsonia - Journal of the Botanical Society of the British Isles 8, 293.
14. Kiernan, J. A. 1972. The involvement of mast cells in vasodilatation due to axon reflexes in injured skin. Quarterly Journal of Experimental Physiology 57, 311-317.
15. Kiernan, J. A. 1972. Effects of known and suspected neurotransmitter substances and of some nucleotides on isolated mast cells. Experientia 28, 653-655.
16. Stoddart, R. W. and Kiernan, J. A. 1973. Histochemical demonstration of the -D-arabinopyranoside configuration with fluorescent-labelled concanavalin A. Histochemie 33 87-94.
17. Kiernan, J. A. and Stoddart, R. W. 1973. Fluorescent-labelled aprotinin: a new reagent for the histochemical detection of acid mucosubstances. Histochemie 34, 77-84.
18. Stoddart, R. W. and Kiernan, J. A. 1973. Aprotinin: a carbohydrate-binding protein. Histochemie 34, 275-280.
19. Drayton, M. R. and Kiernan, J. A. 1973. Effects of aprotinin on organ cultures of the cerebellum of the adult rat. Experimental Neurology 39, 381-388.
20. Cockett, S. A. and Kiernan, J. A. 1973. Acceleration of peripheral nervous regeneration in the rat by exogenous triiodothyronine. Experimental Neurology 39, 389-394.
21. Heap, B. J. and Kiernan, J. A. 1973. Histological, histochemical and pharmacological observations on mast cells in the stomach of the rat. Journal of Anatomy 115, 315-325.
22. Pettit, D. R. and Kiernan, J. A. 1974. Experimental allergic encephalomyelitis in the rat: histopathological studies and the effects of sera on adult cerebellar organ cultures. Acta Neuropathologica 27, 83-91.
23. Kiernan, J. A. 1974. Effects of metabolic inhibitors on vital staining with methylene blue. Histochemistry 40, 51-57.
24. Kiernan, J. A. and Mitchell, R. 1974. Some observations on the innervation of the pinna of the ear of the rat. Journal of Anatomy 117, 397-402.
25. Kiernan, J. A. 1974. Effects of insulin, triiodothyronine and corticosterone on organ cultures of the adult rat cerebellum. Acta Neuropathologica 30, 25-31.
26. Kiernan, J. A. and McIsaac, G. 1974. Complete staining of neuromuscular innervation with bromoindigo and silver. Stain Technology 49, 211-214.
27. Kiernan, J. A. 1974. The development of mast cells in vitro. Journal of Anatomy 118, 517-529.
28. Kiernan, J. A. 1974. Action of adenosine triphosphate on mast cells in normal and denervated skin. Archiv fur Dermatologische Forschung 251, 83-86.
29. Kiernan, J. A. 1975. Localization of -D-glucosyl and -D-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase. Histochemistry 44, 39-45.
30. Kiernan, J. A. 1975. A pharmacological and histological investigation of the involvement of mast cells in cutaneous axon reflex vasodilatation. Quarterly Journal of Experimental Physiology 60, 123-130.
31. McIsaac, G. and Kiernan, J. A. 1975. Accelerated recovery from peripheral nerve injury in experimental hyperthyroidism. Experimental Neurology 48, 88-94.
32. McIsaac, G. and Kiernan, J. A. 1975. Acceleration of neuromuscular reinnervation by triiodothyronine. Journal of Anatomy 120, 551-560.
33. Heap, B. J. and Kiernan, J. A. 1975. Mast cells and gastric secretion in the rat. Quarterly Journal of Experimental Physiology 60, 307-313.
34. Davis, H., Gascho, C. and Kiernan, J. A. 1975. Action of aprotinin on the survival of adult cerebellar neurons in organ culture. Acta Neuropathologica 32, 359-362.
35. Davis, H., Gascho, C, and Kiernan, J. A. 1975. The skin in magnesium-deficient rats. Archives of Dermatological Research 254, 1-13.
36. Davis, H., Gascho, C. and Kiernan, J. A. 1976. Action of aprotinin on organ cultures of the rat's kidney. In Vitro 12, 192-197.
37. Kiernan, J. A. 1976. A comparative survey of the mast cells of the mammalian brain. Journal of Anatomy 121, 303-311.
38. Kiernan, J. A. 1976. Evidence for the involvement of vasoactive constituents of mast cells in axon reflex vasodilatation in the skin of the rat. Archives of Dermatological Research 255, 1-8.
39. Cook, R. A. and Kiernan, J. A. 1976. Effects of triiodothyronine on protein synthesis in regenerating peripheral neurons. Experimental Neurology 52, 515-524.
40. Lampe, M. and Kiernan, J. A. 1976. Degranulation of dermal mast cells: effects of fixation and of antidromic nervous impulses on two histochemically identified cell-types. Archives of Dermatological Research 257, 125-129.
41. Lampe, M. and Kiernan, J. A. 1977. Cytochemical variation of mast cells in the skin of the rat. Archives of Dermatological Research 258, 69-80.
42. Kiernan, J. A. 1977. A study of chemically induced inflammation in the skin of the rat. Quarterly Journal of Experimental Physiology 62, 151-161.
43. Kiernan, J. A. and Heinicke, E. A. 1977. Estimation of lengths of regenerated axons in nerves. Experimental Neurology 56, 431-434.
44. Kiernan, J. A. 1977. Histochemical demonstration of unsaturated hydrophilic lipids with palladium chloride. Journal of Histochemistry and Cytochemistry 25, 200-205.
45. Kiernan, J. A. 1977. Recycling procedure for gold chloride used in neurohistology. Stain Technology 52, 245-248.
46. Stelmack, B. M. and Kiernan, J. A. 1977. Effects of triiodothyronine on the normal and regenerating facial nerve of the rat. Acta Neuropathologica 40, 151-155.
47. Heinicke, E. A. and Kiernan, J. A. 1978. Vascular permeability and axonal regeneration in skin autotransplanted into the brain. Journal of Anatomy 125, 409-420.
48. Mervart, M. and Kiernan, J. A. 1978. Axonal regeneration in experimental allergic peripheral neuritis. Acta Neuropathologica 41, 197-200.
49. Kiernan, J. A. 1978. An explanation of axonal regeneration in peripheral nerves and its failure in the central nervous system. Medical Hypotheses 4, 15-26.
50. Kiernan, J. A. 1978. Recovery of osmium tetroxide from used fixative solutions. Journal of Microscopy 113, 77-82.
51. Kiernan, J. A. 1979. Production and life span of cutaneous mast cells in young rats. Journal of Anatomy 128, 225-238.
52. Sparrow, J. R. and Kiernan, J. A. 1979. Uptake and retrograde transport of proteins by regenerating axons. Acta Neuropathologica 47, 39-47.
53. Kiernan, J. A. 1979. Hypotheses concerned with axonal regeneration in the mammalian nervous system. Biological Reviews 54, 155-197.
54. Davis, H. L. and Kiernan, J. A. 1980. Neurotrophic effects of sciatic nerve extract on denervated extensor digitorum longus muscle in the rat. Experimental Neurology 69, 124-134.
55. Kiernan, J. A. and Contestabile, A. 1980. Vascular permeability associated with axonal regeneration in the optic system of the goldfish. Acta Neuropathologica 51, 39-45.
56. Sparrow, J. R. and Kiernan, J. A. 1981. Endoneurial vascular permeability in degenerating and regenerating peripheral nerves. Acta Neuropathologica 53, 181-188.
57. Davis, H. L. and Kiernan, J. A. 1981. Effect of nerve extract on atrophy of denervated or immobilized muscles. Experimental Neurology 72, 582-591.
58. Doucette, J. R., Kiernan, J. A. and Flumerfelt, B. A. 1983. Two different patterns of retrograde degeneration in the olfactory epithelium following transection of primary olfactory axons. Journal of Anatomy 136, 673-689.
59. Doucette, J. R., Kiernan, J. A. and Flumerfelt, B. A. 1983. The reinnervation of olfactory glomeruli following transection of primary olfactory axons in the central or peripheral nervous system. Journal of Anatomy 137, 1-19.
60. Davis, H. L. and Kiernan, J. A. 1984. Effect of nerve extract on number of acetylcholine receptors in denervated muscles of rats. Experimental Neurology 83, 108-117.
61. Kiernan, J. A. 1984. Chromoxane cyanine R. I. Physical and chemical properties of the dye and of some of its iron complexes. Journal of Microscopy 134, 13-23.
62. Kiernan, J. A. 1984. Chromoxane cyanine R. II. Staining of animal tissues by the dye and its iron complexes. Journal of Microscopy 134, 25-39.
63. Kiernan, J. A. 1985. A conditioning lesion does not promote axonal regeneration in the optic nerve of the rat. Experimental Neurology 87, 181-184.
64. Davis, H. L., Heinicke, E. A., Kiernan, J. A. and Cook, R. A. 1985. Partial purification from mammalian peripheral nerve of a trophic factor which ameliorates atrophy of denervated muscle. Experimental Neurology 89, 159-171.
65. Kiernan, J. A. 1986. The action of chromium(III) in fixation of animal tissues. Histochemical Journal 17, 1131-1146.
66. Kiernan, J. A. 1986. "Pre"-embryos. (Letter, opposing experimentation with human embryos) Nature 321, 376.
67. Kiernan, J. A. 1986. Axonal and vascular changes following injury to the rat's optic nerve. Journal of Anatomy 141, 139-154.
68. Flumerfelt, B. A., Kiernan, J. A., Krcek, J. P. and Sholdice, J. 1986. Reinnervation of skeletal muscle in the tongue by preganglionic parasympathetic vagal neurons. Journal of Anatomy 146, 117-130.
69. Heinicke, E. A., Kiernan, J. A. and Wijsman, J. 1987. Specific, selective and complete staining of neurons of the myenteric plexus, using cuprolinic blue. Journal of Neuroscience Methods 21, 45-54.
70. Kiernan, J. A. 1988. Intestinal anastomosis in the rat facilitated by a rapidly digestible internal splint and indigestible but absorbable sutures. Journal of Surgical Research 45, 427-431.
71. Hudson, A. J. and Kiernan, J. A. 1988. Preservation of certain voluntary muscles in motor neurone disease. (Letter, concerning transneuronal degeneration in MND) Lancet 1988-I, 652.
72. Barr, D. B., Duncan, J. A., Kiernan, J. A., Soper, B. D. and Tepperman, B. L. 1988. Binding and biological actions of prostaglandin E2 and I2 in cells isolated from rabbit gastric mucosa. Journal of Physiology 405, 39-55.
73. Kiernan, J. A. 1989. North American Solidago species in Central England. Proceedings of the Birmingham Natural History Society 26, 22-24.
74. Kiernan, J. A. and Heinicke, E. A. 1989. Sennosides do not kill myenteric neurons in the colon of the rat or mouse. Neuroscience 30, 837-842.
75. Tepperman, B. L., Kiernan, J. A. and Soper, B. D. 1989. The effect of sialoadenectomy on gastric mucosal integrity in the rat: roles of epidermal growth factor and prostaglandin E2. Canadian Journal of Physiology and Pharmacology 67, 1512-1519.
76. Heinicke, E. A. and Kiernan, J. A. 1990. Resistance of myenteric neurons in the rat's colon to depletion by 1,8-dihydroxyanthraquinone. Journal of Pharmacy and Pharmacology 42, 123-125.
77. Heinicke, E. A. and Kiernan, J. A. 1990. An immunohistochemical study of the myenteric plexus of the colon in the rat and mouse. Journal of Anatomy 170, 51-62.
78. Kiernan, J. A. 1990. Degranulation of mast cells in the trachea and bronchi of the rat following stimulation of the vagus nerve. International Archives of Allergy and Applied Immunology 91, 398-402.
79. Kiernan, J. A. 1990. Frozen "pre-embryos". (Letter, deprecating the use of misleading terminology)  JAMA - Journal of the American Medical Association 264, 2283.
80. Kiernan, J. A. 1990. Interactions between mast cells and nerves. Neurogenic inflammation. (Letter, commenting on a recent TIPS review article) Trends in Pharmacological Sciences 11, 316.
81. Allen, D. E. and Kiernan, J. A. 1990. A circulating exogenous protein can enter enteric nervous tissue in the rat. NeuroReport 1, 33-36.
82. Kiernan, J. A. and Hudson, A. J. 1991. Changes in sizes of cortical and lower motor neurons in amyotrophic lateral sclerosis. Brain 114, 843-853.
83. Tepperman, B. L., Besco, J. M., Kiernan, J. A. and Soper, B. D. 1991. Relationship between myeloperoxidase activity and the ontogenic response of rat gastric mucosa to ethanol. Canadian Journal of Physiology and Pharmacology 69, 1882-1888.
84. Pringle, C. E., Hudson, A. J., Munoz, D. G., Kiernan, J. A., Brown, W. F. and Ebers, G. C. 1992. Primary lateral sclerosis. Clinical features, neuropathology and diagnostic criteria. Brain 115, 495-420.
85. Hudson, A. J., Kiernan, J. A., Munoz, D. G., Pringle, C. E., Brown,W. F. and Ebers, G. C. 1993. Clinicopathological features of primary lateral sclerosis are different from amyotrophic lateral sclerosis. Brain Research Bulletin 30, 359-364.
86. Kiernan, J. A. and Hudson, A. J. 1993. Changes in shapes of surviving motor neurons in amyotrophic lateral sclerosis. Brain 116, 203-215.
87. Kiernan, J. A. and Hudson, A. J. 1993. Anti neurone antibodies are not characteristic of amyotrophic lateral sclerosis. NeuroReport 4, 427-430.
88. Allen, D. T. and Kiernan, J. A. 1994. Permeation of proteins from the blood and peripheral nerves into ganglia. Neuroscience 99, 755-764.
89. Kiernan, J. A. and Hudson, A. J. 1994. Frontal lobe atrophy in motor neuron diseases. Brain 117, 747-757.
90. Savedia, S. and Kiernan, J. A. 1994. Increased synthesis of ubiquitin mRNA in motor neurons after axotomy. Neuropathology and Applied Neurobiology 20, 577-586.
91. Kiernan, J. A. 1996. Review of current silver impregnation: Techniques for histological examination of skeletal muscle innervation. Journal of Histotechnology 19, 257-267.
92. Kiernan, J. A. 1996. Vascular permeability in the peripheral autonomic and somatic nervous systems: controversial aspects and comparisons with the blood-brain barrier. Microscopy Research and Technique 35, 122-136.
93. Kiernan, J. A. 1996. Staining paraffin sections without prior removal of the wax. Biotechnic and Histochemistry 71, 304-310.
94. Kiernan, J. A., Macpherson, C. M., Price, A. and Sun, T. 1998. A histochemical examination of the staining of kainate-induced neuronal degeneration by anionic dyes. Biotechnic and Histochemistry 73, 244-254.
95. Conway, K. and Kiernan, J. A. 1999. Chemical dehydration of specimens with 2,2-dimethoxypropane (DMP) for paraffin processing of animal tissues: practical and economic advantages over dehydration in ethanol. Biotechnic and Histochemistry 74, 20-26.
96. Canham, P. B., Finlay, H. M., Kiernan, J. A. and Ferguson, G. G. 1999. Layered structure of saccular aneurysms assessed by collagen birefringence. Neurological Research 21, 618-626.
97. von Bohlen und Halbach, O. and Kiernan, J. A. 1999. Diaminobenzidine induces fluorescence in nervous tissue, providing intrinsic counterstaining of sections prepared for peroxidase histochemistry. Biotechnic and Histochemistry 74, 236-243.
98. Wu, M. & Kiernan, J. A. 2001. A new method for surface staining large slices of fixed brain, using a copper phthalocyanine dye. Biotechnic & Histochemistry 76, 253-255.
99. Kiernan, J. A. 2001. Classification and naming of dyes, stains and fluorochromes. Biotechnic & Histochemistry 76, 261-277.
100. Kiernan, J. A. 2002. Silver staining for spirochetes in tissues: rationale, difficulties, and troubleshooting. Laboratory Medicine 33, 705-708.
101. Kiernan, J. A. 2004. Hexazonium pararosaniline as a fixative for animal tissues. Biotechnic & Histochemistry 79, 203-210.
102. Conway, K., Pin, C. & Kiernan, J. A. & Merrifield, P. 2004. The E-protein HEB is preferentially expressed in developing muscle. Differentiation 72, 327-340.
103. Bova, G. S., Eltoum, I. A., Kiernan, J. A., Siegal, G. P., Frost, A. R., Best, C. J. M., Gillespie, J. W., Su, G. H. & Emmert-Buck, M. R. 2005. Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications. Molecular Biotechnology 29, 119-152.
104. Kiernan, J. A. 2006. Dyes and other colorants in microtechnique and biomedical research. Coloration Technology 122, 1-21.
105. Dapson, R. W., Frank, M. & Kiernan, J. A. 2006. Revised procedures for the certification of carmine (CI 75470, Natural red 4) as a biological stain. Biotechnic & Histochemistry  82: 13-15.
106. Frank, M., Dapson, R. W., Wickersham, T. W. & Kiernan, J. A. 2007. Certification procedures for nuclear fast red (Kernechtrot), C.I. 60760. Biotechnic & Histochemistry  82: 35-39.
107. Kiernan, J. A. 2007. Histochemistry of staining methods for normal and degenerating myelin in the central and peripheral nervous systems. Journal of Histotechnology 30: 87-106.
108. Kiernan, J. A. 2007. Indigogenic substrates for the detection and localization of enzymes.  Biotechnic & Histochemistry 82: 73-103.
109. Kiernan, J. A. 2009. A system for quantitative evaluation of morphological effects of fixatives for light microscopy, using paraffin sections of kidney and brain. Biotechnic & Histochemistry 84: 1-10.
110. Kiernan, J. A. 2009. Graduate training and education in histotechnology and histochemistry. Journal of Histotechnology 32: 115-118.
111.  Dapson, R., Horobin, R. W. & Kiernan, J. A. 2010. Hematoxylin shortages: their causes and duration, and other dyes that can replace hemalum in routine H & E staining. Biotechnic & Histochemistry 85: 55-63.  
112. Dapson, R. W., Fagan, C., Kiernan, J. A. & Wickersham, T. W. 2011. Certification procedures for sirius red F3B (CI 35780, Direct red 80). Biotechnic & Histochemistry 86: 133-139.
113. Kiernan, J. A. 2012. Anatomy of the temporal lobe. Epilepsy Research and Treatment  Vol. 2012: Article ID 176157, 12 pages. doi: 10.1155/2012/176157.
114. Owen, M. A., Schauwers, W., Hugh-Jones, M. E., Kiernan, J. A., Turnbull, P. C. B. & Beyer, W. 2013. A simple, reliable M’Fadyean stain for visualizing the Bacillus anthracis capsule. Journal of Microbiology Methods 92: 264-269.
     

Books

1. Kiernan, J. A. 1981. Histological and Histochemical Methods: Theory and Practice. Oxford: Pergamon Press.
2. Barr, M. L. and Kiernan, J. A. 1983. The Human Nervous System. An Anatomical Viewpoint. 4th edition. Philadelphia: Harper and Row.
3. Kiernan, J. A. 1987. An Introduction to Human Neuroscience. Philadelphia: Lippincott.
4. Barr, M. L. and Kiernan, J. A. 1988. The Human Nervous System. An Anatomical Viewpoint. 5th edition. Philadelphia: Lippincott.
5. Kiernan, J. A. 1990. Histological and Histochemical Methods: Theory and Practice. 2nd edition. Oxford: Pergamon Press.
6. Barr, M. L. and Kiernan, J. A. 1993. The Human Nervous System. An Anatomical Viewpoint. 6th edition. Philadelphia: Lippincott.
7. Kiernan, J. A. 1998. Barr's The Human Nervous System: An Anatomical Viewpoint. 7th edition. Philadelphia: Lippincott-Raven.
8. Kiernan, J. A. 1999. Histological and Histochemical Methods: Theory and Practice. 3rd edition. Oxford: Butterworth-Heinemann. (Paperback edition published in 2000 by Arnold Publishers, London)
9. Horobin, R. W. and Kiernan, J. A. (Eds) 2002. Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for Use in Biology and Medicine. 10th ed. Oxford: BIOS Scientific Publishers (ISBN  1859960995).
10. Kiernan, J. A. and Mason, I. G. (Eds) 2002. Microscopy and Histology for Molecular Biologists. A User's Guide. London: Portland Press (ISBN 1855781417 hard cover, 1855781565 paperback).
11. Kiernan, J. A. 2005. Barr's The Human Nervous System: An Anatomical Viewpoint. 8th edition. Baltimore: Lippincott, Williams & Wilkins.
12. Kiernan, J. A. 2008. Histological and Histochemical Methods: Theory and Practice. 4th edition. Bloxham, UK: Scion Publishing. (ISBN 9781904842422)
13. Kiernan, J. A. 2009. Barr's The Human Nervous System: An Anatomical Viewpoint. 9th edition. Baltimore: Lippincott, Williams & Wilkins (ISBN 9780781782562).
14. Kumar, G. L. and Kiernan, J. A. (Eds) 2010. Education Guide: Special Stains and H & E. 2nd ed. Carpinteria, CA: Dako North America. 300 pages. Printed book available on request from LitReqUS@dako.com or download from either http://www.dako.com/us/08066_special_stains_eduguide.pdf (whole book) or http://www.dako.com/us/index/knowledgecenter/kc_publications_edu/special_stains.htm (individual chapters).
15. Kiernan, J. A. & Rajakumar, N. 2013.  Barr's The Human Nervous System: An Anatomical Viewpoint. 10th edition. Baltimore: Lippincott, Williams & Wilkins (in press)

Chapters in books

1. Kiernan, J. A. & Berry, M. 1975. Neuroanatomical methods. Chapter 1 in Methods in Brain Research, ed. P. B. Bradley. London: John Wiley. pp. 1-77.
2. Kiernan, J. A. 1984. Role of mast cells in vascular responses due to axon reflexes in injured skin. In Antidromic Vasodilatation and Neurogenic Inflammation, ed. L. A. Chahl, J. Szolczanyi and F. Lembeck. Budapest: Akademiai Kiado. pp. 223-243.
3. Kiernan, J. A. 1992. Some functional pathways in the central nervous system. Chapter 450 in Textbook of Internal Medicine, 2nd ed., ed. W. N. Kelley, Vol. 2, pp. 2138-2145. Philadelphia: Lippincott.
4. Kiernan, J. A. 1997. Some functional pathways in the central nervous system. Chapter 17 in Textbook of Internal Medicine, 3rd ed., ed. W. N. Kelley, pp. 94-102. Philadelphia: Lippincott-Raven.
5. Kiernan, J. A. 2000. Some functional pathways in the central nervous system. Chapter 14 in Kelley's Textbook of Internal Medicine, 4th ed., ed. H. D. Humes, pp. 99-108. Philadelphia: Lippincott, Williams and Wilkins.
6. Kiernan, J. A. 2002. Freezing and fixation. Chapter 8 in Microscopy and Histology for Molecular Biologists. A User's Guide, ed. Kiernan, J. A. & Mason, I. G. London: Portland Press.
7. Kiernan, J. A. 2002. Staining and counterstaining to show structural features. Chapter 10 in Microscopy and Histology for Molecular Biologists. A User's Guide, ed. Kiernan, J. A. & Mason, I. G. London: Portland Press.
8. Kiernan, J. A. 2002. Nomenclature and classification of dyes and other coloring agents. Chapter 3 in Horobin, R. W. & Kiernan, J. A. (eds) Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for use in Biology and Medicine. 10th ed. Oxford: BIOS. pp. 23-39.
9. Kiernan, J. A. & Horobin, R. W. 2002. Miscellaneous inorganic and organic substances used as biological stains. Chapter 27 in Horobin, R. W. & Kiernan, J. A. (eds) Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for use in Biology and Medicine. 10th ed. Oxford: BIOS. pp. 389-415.
10. Bova, G. S., Eltoum, I. A., Kiernan, J. A., Siegal, G. P., Frost, A. R., Best, C. J. M., Gillespie, J. W. & Emmert-Buck, M. R. 2004. Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications. Ch. 2 in Su, G. H. ed. Pancreatic Cancer. Methods and Protocols. (Methods in Molecular Medicine, Vol. 103). Totowa, NJ: Humana Press. pp. 15-66. (Also as an e-Book: http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-780-7&txtSearchStartPos=1)

Other publications

1. Kiernan, J. A. 1965. Some histochemical observations relating to the so-called ground substance of the central nervous system. Journal of Anatomy 99, 926-928.
2. Kiernan, J. A. 1965. Application of a fluorescent periodic acid-Schiff technique to the central nervous system. Journal of Anatomy 99, 940.
3. Kiernan, J. A. 1970. Two types of axonal regeneration in the neurohypophysis of the rat. Journal of Anatomy 107, 187.
4. Kiernan, J. A. 1971. Subcellular studies of axonal argyrophilia. Journal of Anatomy 108, 623.
5. Kiernan, J. A. 1996. Murray Llewellyn Barr (1908-1995). (Obituary) Journal of Anatomy 188, 747-748.
6. Kiernan, J. A. 1997. Making and using aqueous mounting media. Microscopy Today 97-10, 16-17.
7. Kiernan, J. A. 1998. Double embedding in cellulose nitrate and paraffin wax, an old and useful method that is often misunderstood. Microscopy Today 98-2, 12-14.
8. Kiernan, J. A. 1999. Long-lasting aqueous mounts for light microscopy. Microscopy Today 99-1, 3.
9. Kiernan, J. A. 1999. Strategies for preventing detachment of sections from glass slides. Microscopy Today 99-6, 22-24.
10. Kiernan, J. A. 1999. Recycling gold. Microscopy Today 99-10, 23-25.
11. Kiernan, J. A. 1999. Staining and microscopy: the need for old and new methods. (Guest editorial) Biotechnic and Histochemistry 74, 275.
12. Kiernan, J. A. 2000. Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: What they are and what they do. Microscopy Today 00-1, 8-12.
13. Kiernan, J. A. 2000. Rit scarlet No. 5 as a stain for amyloid. (Letter, commenting on paper in J. Histotechnol. 22: [1] 33-35, 1999) Journal of Histotechnology 23, 87.
14. Kiernan, J. A. 2000. Preventing lost tissues: a look at charged slides. What are the exact differences between poly-L-lysine and aminoalkyloxysilanes? Paraffin Press (Connecticut Society of Histotechnologists) Spring 2000, 3.
15. Kiernan, J. A. 2000. Dehydrating delicate specimens for light microscopy. Microscopy Today 00-7, 37.
16. Kiernan, J. A. 2001. How to stain motor end plates. Microscopy Today 01-5: 29.
17. Kiernan, J. A. 2001. How acids and chelating agents soften bones and teeth. Hard times. Communiqué‚ of the Hard Tissue Committee, National Society for Histotechnology 13 (2) July 2001. (mini-review article)
18. Kiernan, J. A., Pyle, R. H. & Silverman, J. S. 2001. N.S.H. Self Assessment Examinations, Series 5: Immunohistochemistry, Enzyme Histochemistry, Flow Cytometry, In-Situ Hybridization, Electron Microscopy. 2nd ed. Bowie, MD, National Society for Histotechnology.
19. Kiernan, J. A. (section editor) 2002- . Notes and Queries. A regular feature comprising peer-reviewed answers to readers' questions. Biotechnic & Histochemistry Vol. 76 onwards.
20. Kiernan, J. A. 2005. Preservation and retrieval of antigens for immunohistochemistry - methods and mechanisms. 1. Effects of formaldehyde fixation. The Cutting Edge January 2005, pp. 5-9. (National Society for Histotechnology, Newsletter for Region IX, Canada)
21. Kiernan, J. A. 2005. Preservation and retrieval of antigens for immunohistochemistry - methods and mechanisms. Part 2. Retrieving masked antigens. The Cutting Edge April 2005, pp. 5-11. (National Society for Histotechnology, Newsletter for Region IX, Canada)
22. Kiernan, J. A. 2005. Nissl: the man, the stain and the substance. Microscopy Today 13(6), 50.
23. Kiernan, J. A. & Sims, S. M. 2006. Preface,  in Hudson, A. J. (2006) The Physiological Basis of Memory and Consciousness. Lewiston, NY: Edwin Mellen Press.
24. Lyon, H. O. & Kiernan, J. A. 2008. News from the Biological Stain Commission.  Biotechnic & Histochemistry 83: 201-203.
25. Lyon, H. O. & Kiernan, J. A. 2008. News from the Biological Stain Commission.  Biotechnic & Histochemistry 83: 285-288.
26. Kiernan, J. A. & Horobin, R. W. 2010. A special issue devoted to hematoxylin, hematein and hemalum. Biotechnic & Histochemistry 85: 5-6. (Editorial).
27. Kumar, G. L. & Kiernan, J. A. 2010. Editorial. Special stains and H&E. Connection (Dako Inc. Scientific Magazine). Vol. 14, p. 14.
28. Kiernan, J. A. 2010. Staining sections of the central nervous system. Connection (Dako Inc. Scientific Magazine). Vol. 14, pp. 60-67.
29. Kiernan, J. A. 2010. Carbohydrate histochemistry. Connection (Dako Inc. Scientific Magazine). Vol. 14, pp. 68-78.
30. Kiernan, J. A. 2010. On chemical reactions and staining mechanisms. Connection (Dako Inc. Scientific Magazine). Vol. 14, pp. 127-136. [What is the difference between argentaffin and argyrophilic reactions? (p. 127-128). How does pH influence the staining mechanism of Schiff's reagent in biological tissue samples? (p. 129-131). What is Giemsa’s stain and how does it color blood cells, bacteria and chromosomes? (p. 132-136).]
31. Kiernan, J. A. 2013. Editorial.  Journal of Histotechnology 36(1): 1-2.  DOI: 10.1179/0147888513Z.00000000041 (Summary and brief discussion of papers in the March 2013 issue of the journal).

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Last updated:  March 2013