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Some information about me    (Mostly pretty boring stuff)    Click here to skip down to research and publications

Name: John Alan Kiernan.
Born: 1942; Kidderminster, Worcestershire, England.
Married: 1967 to Tessa Mary Westcott; 5 children; 11 grandchildren.
Job(s): Retired from teaching neuroanatomy to medical and other students. Research in neuroscience and in developing and improving histological and histochemical techniques. Occasional consultant for medical devices companies, patent law firms, publishers and universities, mostly in USA, also Asia.  Secretary and a trustee  of the Biological Stain Commission.
Resides in:  St Thomas, Ontario, Canada.  (This is half-way between London and  the North Shore of Lake Erie, and roughly equidistant from Toronto, Buffalo and Detroit, all about a 150 minutes drive away.)  
Other interests: Field botany (vascular plants).
Mugshot: JK2018.jpg


Education:

1953-1960: King Edward VI Grammar School, Five Ways, Birmingham, England.
1960-1966 and 1967-1969: University of Birmingham, England.

Degrees etc.

1963: BSc (Anatomical Studies) University of Birmingham, England.
1966: MB, ChB University of Birmingham, England.
1966: MRCS England; LRCP London.
1969: PhD (Faculty of Medicine) University of Birmingham, England.  Thesis: Studies on Nervous Regeneration
1969: MA (conferred by statute as a condition of appointment) University of Cambridge, England.
1979: DSc (by thesis, Studies in Neurohistology and Related Fields, through Department of Anatomy) University of Birmingham, England.

Employment:

1966:   House Surgeon, Accident Unit, East Birmingham Hospital, Birmingham, England.
1967:   House Physician, Worcester Royal Infirmary, Worcester, England.

1967-1969:   Research Fellow (MRC), Department of Anatomy, University of Birmingham, England.

1967-1972:   General medical practice, part time, Birmingham, Cambridge etc.

1969-1971:   Stanley Elmore Senior Research Fellow, Sidney Sussex College, Cambridge, England.

1970-1972:   Director of Studies in Medicine, Sidney Sussex College, Cambridge, England.

1971-1972:   University Demonstrator, Department of Anatomy, University of Cambridge; College Lecturer and Fellow, Sidney Sussex College, Cambridge, England.
1972-1975:   Assistant Professor, Department of Anatomy, The University of Western Ontario, London, Canada.
1975-1981:   Associate Professor, Department of Anatomy, The University of Western Ontario (Tenure, 1975).
1981-2011:   Professor, Department of Anatomy, The University of Western Ontario, London, Canada. 
     1985-1986:   Commonwealth Medical Fellow, and visiting professor, Department of  Anatomy and Cell Biology, The University of Sheffield, England.
     1990-2011:   Core faculty member, Neuroscience Graduate Programme, The University of Western Ontario, London, Canada.
2011-        :   Professor Emeritus, Department of Anatomy & Cell Biology, The University of Western Ontario, London, Canada.

Membership of societies, editorial boards etc.

Undergraduate teaching (University of  Birmingham)

Undergraduate teaching (University of Cambridge)

Undergraduate teaching (University of Western Ontario):

Graduate courses taught (University of Western Ontario):


Research interests:

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Publications Peer reviewed papers
Books
Chapters
Other publications

Items are colour-coded by subject:

A note on multiple authorship of papers in peer-reviewed journals. Most of the publications listed in my CV have 1, 2 or3 authors, all equal contributors by virtue of supervision, doing the lab work and producing the manuscript. Usually the first author did most of the lab work and the last author was the supervisor who wrote the paper and responded to the referees' reports. For some papers in this list, especially before about 1990, the authors are listed alphabetically. This was an admirable principle held by the Journal of Physiology for several decades, which I tried to encourage among collaborators in papers submitted to all journals. (I'm an author of only one paper in J. Physiol.; I did some of the lab work. Happily the supervisor of the project had a surname beginning with T and retained his last-author position.) I support the notion that authors should be equal contributors, whereas minor contributions (academic, financial, technical etc) should be recognized in acknowledgements.
There is no generally agreed formula that determines the order in a list of "authors" of a scientific paper. I appear as a "middle" author in some of the following items, including at least three for which I deserve only an  acknowledgement.  For a few, I'm in the middle of a list of three, having supervised two students with surnames before and after K in the alphabet.

Papers in peer-reviewed journals

  1. Holmes, R. L. and Kiernan, J. A. 1964. The fine structure of the infundibular process of the hedgehog. Zeitschrift fur Zellforschung 61, 894-912.  https://doi.org/10.1007/BF00340042
  2. Kiernan, J. A. 1964. Carboxylic esterases of the hypothalamus and neurohypophysis of the hedgehog. Journal of the Royal Microscopical Society 83, 297-306. 
  3. Campbell, D. J. and Kiernan, J. A. 1966. Mast cells in the central nervous system. Nature 210, 756-757.  https://doi.org/10.1038/210756b0
  4. Kiernan, J. A. 1966. Notes on the flora of Worcestershire: additions to the flora since 1909. Proceedings of the Birmingham Natural History Society 20, 19-37. 
  5. Kiernan, J. A. 1967. On the probable absence of retino-hypothalamic connections in five mammals and an amphibian. Journal of Comparative Neurology 131, 405-408. 
  6. Kiernan, J. A. 1968. Effect of interrupting retino-hypothalamic connections on the melanophore response in Xenopus laevis. Experientia 24, 503-504. https://doi.org/10.1007/BF02144419
  7. Kiernan, J. A. 1968. Notes on the flora of Worcestershire. II. Further additions to the flora since 1909. Proceedings of the Birmingham Natural History Society 21, 83-88.
  8. Kiernan, J. A. 1970. Silver staining of axons in subcellular fractions of nervous tissue. Experientia 26, 1352.  https://doi.org/10.1007/BF02113026
  9. Kiernan, J. A. 1971. Pituicytes and the regenerative properties of neurosecretory and other axons in the rat. Journal of Anatomy 109, 97-114. 
    PMCID: PMC1270967.
  10. Kiernan, J. A. and Rawcliffe, P. M. 1971. Effects of triiodothyronine on the cerebellar cortex of the new-born rat in tissue culture. Experientia 27, 678-679.  https://doi.org/10.1007/BF02136957
  11. Kiernan, J. A. and Pettit, D. R. 1971. Organ culture of the central nervous system of the adult rat. Experimental Neurology 32, 111-120.  https://doi.org/10.1016/0014-4886(71)90169-5
  12. Fertig, A., Kiernan, J. A. and Seyan, S. S. A. S. 1971. Enhancement of axonal regeneration in the brain of the rat by corticotrophin and triiodothyronine. Experimental Neurology 33, 372-385.  https://doi.org/10.1016/0014-4886(71)90029-X
  13. Kiernan, J. A. 1971. Rhynchosinapis - The Worcestershire records. Watsonia - Journal of the Botanical Society of the British Isles 8, 293.
  14. Kiernan, J. A. 1972. The involvement of mast cells in vasodilatation due to axon reflexes in injured skin. Quarterly Journal of Experimental Physiology 57, 311-317.  
  15. Kiernan, J. A. 1972. Effects of known and suspected neurotransmitter substances and of some nucleotides on isolated mast cells. Experientia 28, 653-655.  https://doi.org/10.1007/BF01944958
  16. Stoddart, R. W. and Kiernan, J. A. 1972. Histochemical demonstration of the α-D-arabinopyranoside configuration with fluorescent-labelled concanavalin A. Histochemie 33 87-94.  https://doi.org/10.1007/BF00304230
  17. Kiernan, J. A. and Stoddart, R. W. 1973. Fluorescent-labelled aprotinin: a new reagent for the histochemical detection of acid mucosubstances. Histochemie 34, 77-84. https://doi.org/10.1007/BF00304309
  18. Stoddart, R. W. and Kiernan, J. A. 1973. Aprotinin: a carbohydrate-binding protein. Histochemie 34, 275-280.  https://doi.org/10.1007/BF00306299
  19. Drayton, M. R. and Kiernan, J. A. 1973. Effects of aprotinin on organ cultures of the cerebellum of the adult rat. Experimental Neurology 39, 381-388.  https://doi.org/10.1016/0014-4886(73)90024-1
  20. Cockett, S. A. and Kiernan, J. A. 1973. Acceleration of peripheral nervous regeneration in the rat by exogenous triiodothyronine. Experimental Neurology 39, 389-394.  https://doi.org/10.1016/0014-4886(73)90025-3
  21. Heap, B. J. and Kiernan, J. A. 1973. Histological, histochemical and pharmacological observations on mast cells in the stomach of the rat. Journal of Anatomy 115, 315-325.  
    PMCID: PMC1271487
  22. Pettit, D. R. and Kiernan, J. A. 1974. Experimental allergic encephalomyelitis in the rat: histopathological studies and the effects of sera on adult cerebellar organ cultures. Acta Neuropathologica 27, 83-91.  https://doi.org/10.1007/BF00687243
  23. Kiernan, J. A. 1974. Effects of metabolic inhibitors on vital staining with methylene blue. Histochemistry 40, 51-57.  https://doi.org/10.1007/BF00490273
  24. Kiernan, J. A. and Mitchell, R. 1974. Some observations on the innervation of the pinna of the ear of the rat. Journal of Anatomy 117, 397-402.  PMCID: PMC1231412
  25. Kiernan, J. A. 1974. Effects of insulin, triiodothyronine and corticosterone on organ cultures of the adult rat cerebellum. Acta Neuropathologica 30, 25-31.  https://doi.org/10.1007/BF00685319
  26. Kiernan, J. A. and McIsaac, G. 1974. Complete staining of neuromuscular innervation with bromoindigo and silver. Stain Technology 49, 211-214.  https://doi.org/10.3109/10520297409116980
  27. Kiernan, J. A. 1974. The development of mast cells in vitro. Journal of Anatomy 118, 517-529.  PMCID: PMC1231550
  28. Kiernan, J. A. 1974. Action of adenosine triphosphate on mast cells in normal and denervated skin. Archiv fur Dermatologische Forschung 251, 83-86.  https://doi.org/10.1007/BF00561714
  29. Kiernan, J. A. 1975. Localization of α-D-glucosyl and α-D-mannosyl groups of mucosubstances with concanavalin A and horseradish peroxidase. Histochemistry 44, 39-45.  https://doi.org/10.1007/BF00490419
  30. Kiernan, J. A. 1975. A pharmacological and histological investigation of the involvement of mast cells in cutaneous axon reflex vasodilatation. Quarterly Journal of Experimental Physiology 60, 123-130. https://doi.org/10.1113/expphysiol.1975.sp002298  
  31. McIsaac, G. and Kiernan, J. A. 1975. Accelerated recovery from peripheral nerve injury in experimental hyperthyroidism. Experimental Neurology 48, 88-94.  https://doi.org/10.1016/0014-4886(75)90224-1
  32. McIsaac, G. and Kiernan, J. A. 1975. Acceleration of neuromuscular reinnervation by triiodothyronine. Journal of Anatomy 120, 551-560.  PMCID: PMC1231695
  33. Heap, B. J. and Kiernan, J. A. 1975. Mast cells and gastric secretion in the rat. Quarterly Journal of Experimental Physiology 60, 307-313.  
  34. Davis, H., Gascho, C. and Kiernan, J. A. 1975. Action of aprotinin on the survival of adult cerebellar neurons in organ culture. Acta Neuropathologica 32, 359-362.  https://doi.org/10.1007/BF00696799
  35. Davis, H., Gascho, C, and Kiernan, J. A. 1975. The skin in magnesium-deficient rats. Archives of Dermatological Research 254, 1-13.  https://doi.org/10.1007/BF00561528
  36. Davis, H., Gascho, C. and Kiernan, J. A. 1976. Action of aprotinin on organ cultures of the rat's kidney. In Vitro 12, 192-197.  https://doi.org/10.1007/BF02796441
  37. Kiernan, J. A. 1976. A comparative survey of the mast cells of the mammalian brain. Journal of Anatomy 121, 303-311.  PMCID: PMC1231801  
  38. Kiernan, J. A. 1976. Evidence for the involvement of vasoactive constituents of mast cells in axon reflex vasodilatation in the skin of the rat. Archives of Dermatological Research 255, 1-8.  https://doi.org/10.1007/BF00581672
  39. Cook, R. A. and Kiernan, J. A. 1976. Effects of triiodothyronine on protein synthesis in regenerating peripheral neurons. Experimental Neurology 52, 515-524.  https://doi.org/10.1016/0014-4886(76)90222-3
  40. Lampe, M. and Kiernan, J. A. 1976. Degranulation of dermal mast cells: effects of fixation and of antidromic nervous impulses on two histochemically identified cell-types. Archives of Dermatological Research 257, 125-129.  https://doi.org/10.1007/BF00558085
  41. Lampe, M. and Kiernan, J. A. 1977. Cytochemical variation of mast cells in the skin of the rat. Archives of Dermatological Research 258, 69-80.  https://doi.org/10.1007/BF00582869
  42. Kiernan, J. A. 1977. A study of chemically induced inflammation in the skin of the rat. Quarterly Journal of Experimental Physiology 62, 151-161.  
  43. Kiernan, J. A. and Heinicke, E. A. 1977. Estimation of lengths of regenerated axons in nerves. Experimental Neurology 56, 431-434.  https://doi.org/10.1016/0014-4886(77)90359-4
  44. Kiernan, J. A. 1977. Histochemical demonstration of unsaturated hydrophilic lipids with palladium chloride. Journal of Histochemistry and Cytochemistry 25, 200-205.  
  45. Kiernan, J. A. 1977. Recycling procedure for gold chloride used in neurohistology. Stain Technology 52, 245-248.  https://doi.org/10.3109/10520297709116787
  46. Stelmack, B. M. and Kiernan, J. A. 1977. Effects of triiodothyronine on the normal and regenerating facial nerve of the rat. Acta Neuropathologica 40, 151-155. https://doi.org/10.1007/BF00688704 
  47. Heinicke, E. A. and Kiernan, J. A. 1978. Vascular permeability and axonal regeneration in skin autotransplanted into the brain. Journal of Anatomy 125, 409-420. PMCID: PMC342472  
  48. Mervart, M. and Kiernan, J. A. 1978. Axonal regeneration in experimental allergic peripheral neuritis. Acta Neuropathologica 41, 197-200.  https://doi.org/10.1007/BF00690435
  49. Kiernan, J. A. 1978. An explanation of axonal regeneration in peripheral nerves and its failure in the central nervous system. Medical Hypotheses 4, 15-26.  https://doi.org/10.1016/0306-9877(78)90022-1
  50. Kiernan, J. A. 1978. Recovery of osmium tetroxide from used fixative solutions. Journal of Microscopy 113, 77-82.  
  51. Kiernan, J. A. 1979. Production and life span of cutaneous mast cells in young rats. Journal of Anatomy 128, 225-238.  PMCID: PMC1232929  
  52. Sparrow, J. R. and Kiernan, J. A. 1979. Uptake and retrograde transport of proteins by regenerating axons. Acta Neuropathologica 47, 39-47.  https://doi.org/10.1007/BF00698271
  53. Kiernan, J. A. 1979. Hypotheses concerned with axonal regeneration in the mammalian nervous system. Biological Reviews 54, 155-197. 
  54. Davis, H. L. and Kiernan, J. A. 1980. Neurotrophic effects of sciatic nerve extract on denervated extensor digitorum longus muscle in the rat. Experimental Neurology 69, 124-134.  https://doi.org/10.1016/0014-4886(80)90148-X
  55. Kiernan, J. A. and Contestabile, A. 1980. Vascular permeability associated with axonal regeneration in the optic system of the goldfish. Acta Neuropathologica 51, 39-45.  https://doi.org/10.1007/BF00688848
  56. Sparrow, J. R. and Kiernan, J. A. 1981. Endoneurial vascular permeability in degenerating and regenerating peripheral nerves. Acta Neuropathologica 53, 181-188.  https://doi.org/10.1007/BF00688020
  57. Davis, H. L. and Kiernan, J. A. 1981. Effect of nerve extract on atrophy of denervated or immobilized muscles. Experimental Neurology 72, 582-591.  https://doi.org/10.1016/0014-4886(81)90007-8
  58. Doucette, J. R., Kiernan, J. A. and Flumerfelt, B. A. 1983. Two different patterns of retrograde degeneration in the olfactory epithelium following transection of primary olfactory axons. Journal of Anatomy 136, 673-689.  PMCID: PMC6886621  
  59. Doucette, J. R., Kiernan, J. A. and Flumerfelt, B. A. 1983. The reinnervation of olfactory glomeruli following transection of primary olfactory axons in the central or peripheral nervous system. Journal of Anatomy 137, 1-19.  PMCID: PMC6630027  
  60. Davis, H. L. and Kiernan, J. A. 1984. Effect of nerve extract on number of acetylcholine receptors in denervated muscles of rats. Experimental Neurology 83, 108-117.  https://doi.org/10.1016/0014-4886(84)90050-5
  61. Kiernan, J. A. 1984. Chromoxane cyanine R. I. Physical and chemical properties of the dye and of some of its iron complexes. Journal of Microscopy 134, 13-23.  
  62. Kiernan, J. A. 1984. Chromoxane cyanine R. II. Staining of animal tissues by the dye and its iron complexes. Journal of Microscopy 134, 25-39.  
  63. Kiernan, J. A. 1985. A conditioning lesion does not promote axonal regeneration in the optic nerve of the rat. Experimental Neurology 87, 181-184.  https://doi.org/10.1016/0014-4886(85)90145-1
  64. Davis, H. L., Heinicke, E. A., Kiernan, J. A. and Cook, R. A. 1985. Partial purification from mammalian peripheral nerve of a trophic factor which ameliorates atrophy of denervated muscle. Experimental Neurology 89, 159-171.  https://doi.org/10.1016/0014-4886(85)90273-0
  65. Kiernan, J. A. 1986. The action of chromium(III) in fixation of animal tissues. Histochemical Journal 17, 1131-1146.  https://doi.org/10.1007/BF01002538
  66. Kiernan, J. A. 1986. "Pre"-embryos. (Letter, opposing experimentation with human embryos) Nature 321, 376.  https://doi.org/10.1038/321376b0
  67. Kiernan, J. A. 1986. Axonal and vascular changes following injury to the rat's optic nerve. Journal of Anatomy 141, 139-154.  PMCID:  PMC1166396  
  68. Flumerfelt, B. A., Kiernan, J. A., Krcek, J. P. and Sholdice, J. 1986. Reinnervation of skeletal muscle in the tongue by preganglionic parasympathetic vagal neurons. Journal of Anatomy 146, 117-130.  PMCID:  PMC1166528  
  69. Heinicke, E. A., Kiernan, J. A. and Wijsman, J. 1987. Specific, selective and complete staining of neurons of the myenteric plexus, using cuprolinic blue. Journal of Neuroscience Methods 21, 45-54.  https://doi.org/10.1016/0165-0270(87)90101-4
  70. Kiernan, J. A. 1988. Intestinal anastomosis in the rat facilitated by a rapidly digestible internal splint and indigestible but absorbable sutures. Journal of Surgical Research 45, 427-431.  https://doi.org/10.1016/0022-4804(88)90192-8
  71. Hudson, A. J. and Kiernan, J. A. 1988. Preservation of certain voluntary muscles in motor neurone disease. (Letter, concerning transneuronal degeneration in MND) Lancet 1988-I, 331: 652-653.  https://doi.org/10.1016/S0140-6736(88)91455-9
  72. Barr, D. B., Duncan, J. A., Kiernan, J. A., Soper, B. D. and Tepperman, B. L. 1988. Binding and biological actions of prostaglandin E2 and I2 in cells isolated from rabbit gastric mucosa. Journal of Physiology 405, 39-55. https://doi.org/10.1113/jphysiol.1988.sp017320 
  73. Kiernan, J. A. 1989. North American Solidago species in Central England. Proceedings of the Birmingham Natural History Society 26, 22-24.
  74. Kiernan, J. A. and Heinicke, E. A. 1989. Sennosides do not kill myenteric neurons in the colon of the rat or mouse. Neuroscience 30, 837-842.  https://doi.org/10.1016/0306-4522(89)90175-9 
  75. Tepperman, B. L., Kiernan, J. A. and Soper, B. D. 1989. The effect of sialoadenectomy on gastric mucosal integrity in the rat: roles of epidermal growth factor and prostaglandin E2. Canadian Journal of Physiology and Pharmacology 67, 1512-1519.  https://doi.org/10.1139/y89-244https://doi.org/10.1139/y89-244
  76. Heinicke, E. A. and Kiernan, J. A. 1990. Resistance of myenteric neurons in the rat's colon to depletion by 1,8-dihydroxyanthraquinone. Journal of Pharmacy and Pharmacology 42, 123-125.  https://doi.org/10.1111/j.2042-7158.1990.tb05366.x
  77. Heinicke, E. A. and Kiernan, J. A. 1990. An immunohistochemical study of the myenteric plexus of the colon in the rat and mouse. Journal of Anatomy 170, 51-62.  PMCID:  PMC1257062  
  78. Kiernan, J. A. 1990. Degranulation of mast cells in the trachea and bronchi of the rat following stimulation of the vagus nerve. International Archives of Allergy and Applied Immunology 91, 398-402.  https://doi.org/10.1159/000235149
  79. Kiernan, J. A. 1990. Frozen "pre-embryos". (Letter, deprecating the use of misleading terminology)  JAMA - Journal of the American Medical Association 264, 2283. https://doi.org/10.1001/jama.1990.03450180038014
  80. Kiernan, J. A. 1990. Interactions between mast cells and nerves. Neurogenic inflammation. (Letter, commenting on a recent TIPS review article) Trends in Pharmacological Sciences 11, 316.  PDF download link
  81. Allen, D. E. and Kiernan, J. A. 1990. A circulating exogenous protein can enter enteric nervous tissue in the rat. NeuroReport 1, 33-36. https://doi.org/10.1097/00001756-199009000-00010 
  82. Kiernan, J. A. and Hudson, A. J. 1991. Changes in sizes of cortical and lower motor neurons in amyotrophic lateral sclerosis. Brain 114, 843-853.  https://doi.org/10.1093/brain/114.2.843
  83. Tepperman, B. L., Besco, J. M., Kiernan, J. A. and Soper, B. D. 1991. Relationship between myeloperoxidase activity and the ontogenic response of rat gastric mucosa to ethanol. Canadian Journal of Physiology and Pharmacology 69, 1882-1888.  https://doi.org/10.1139/y91-278
  84. Pringle, C. E., Hudson, A. J., Munoz, D. G., Kiernan, J. A., Brown, W. F. and Ebers, G. C. 1992. Primary lateral sclerosis. Clinical features, neuropathology and diagnostic criteria. Brain 115, 495-420. https://doi.org/10.1093/brain/115.2.495
  85. Hudson, A. J., Kiernan, J. A., Munoz, D. G., Pringle, C. E., Brown,W. F. and Ebers, G. C. 1993. Clinicopathological features of primary lateral sclerosis are different from amyotrophic lateral sclerosis. Brain Research Bulletin 30, 359-364. https://doi.org/10.1016/0361-9230(93)90265-D
  86. Kiernan, J. A. and Hudson, A. J. 1993. Changes in shapes of surviving motor neurons in amyotrophic lateral sclerosis. Brain 116, 203-215. https://doi.org/10.1093/brain/116.1.203
  87. Kiernan, J. A. and Hudson, A. J. 1993. Anti neurone antibodies are not characteristic of amyotrophic lateral sclerosis. NeuroReport 4, 427-430. https://doi.org/
  88. Allen, D. T. and Kiernan, J. A. 1994. Permeation of proteins from the blood and peripheral nerves into ganglia. Neuroscience 99, 755-764.  https://doi.org/10.1016/0306-4522(94)90192-9
  89. Kiernan, J. A. and Hudson, A. J. 1994. Frontal lobe atrophy in motor neuron diseases. Brain 117, 747-757. https://doi.org/10.1093/brain/117.4.747
  90. Savedia, S. and Kiernan, J. A. 1994. Increased synthesis of ubiquitin mRNA in motor neurons after axotomy. Neuropathology and Applied Neurobiology 20, 577-586. 
  91. Kiernan, J. A. 1996. Review of current silver impregnation: Techniques for histological examination of skeletal muscle innervation. Journal of Histotechnology 19, 257-267.
  92. Kiernan, J. A. 1996. Vascular permeability in the peripheral autonomic and somatic nervous systems: controversial aspects and comparisons with the blood-brain barrier. Microscopy Research and Technique 35, 122-136. https://doi.org/10.1002/(SICI)1097-0029(19961001)35:2<122::AID-JEMT3>3.0.CO;2-S
  93. Kiernan, J. A. 1996. Staining paraffin sections without prior removal of the wax. Biotechnic and Histochemistry 71, 304-310. https://doi.org/10.3109/10520299609117180
  94. Kiernan, J. A., Macpherson, C. M., Price, A. and Sun, T. 1998. A histochemical examination of the staining of kainate-induced neuronal degeneration by anionic dyes. Biotechnic and Histochemistry 73, 244-254. https://doi.org/10.3109/10520299809141118
  95. Conway, K. and Kiernan, J. A. 1999. Chemical dehydration of specimens with 2,2-dimethoxypropane (DMP) for paraffin processing of animal tissues: practical and economic advantages over dehydration in ethanol. Biotechnic and Histochemistry 74, 20-26. https://doi.org/10.3109/10520299909066473
  96. Canham, P. B., Finlay, H. M., Kiernan, J. A. and Ferguson, G. G. 1999. Layered structure of saccular aneurysms assessed by collagen birefringence. Neurological Research 21, 618-626. https://doi.org/10.1080/01616412.1999.11740986
  97. von Bohlen und Halbach, O. and Kiernan, J. A. 1999. Diaminobenzidine induces fluorescence in nervous tissue, providing intrinsic counterstaining of sections prepared for peroxidase histochemistry. Biotechnic and Histochemistry 74, 236-243. https://doi.org/10.3109/10520299909034659
  98. Wu, M. & Kiernan, J. A. 2001. A new method for surface staining large slices of fixed brain, using a copper phthalocyanine dye. Biotechnic & Histochemistry 76, 253-255. https://doi.org/10.1080/10520290802475878
  99. Kiernan, J. A. 2001. Classification and naming of dyes, stains and fluorochromes. Biotechnic & Histochemistry 76, 261-277. https://doi.org/10.1080/bih.76.5-6.261.278
  100. Kiernan, J. A. 2002. Silver staining for spirochetes in tissues: rationale, difficulties, and troubleshooting. Laboratory Medicine 33, 705-708. Click here for link to PDF file
  101. Kiernan, J. A. 2004. Hexazonium pararosaniline as a fixative for animal tissues. Biotechnic & Histochemistry 79, 203-210. https://doi.org/10.1080/10520290400018005
  102. Conway, K., Pin, C. & Kiernan, J. A. & Merrifield, P. 2004. The E-protein HEB is preferentially expressed in developing muscle. Differentiation 72, 327-340. https://doi.org/10.1111/j.1432-0436.2004.07207004.x 
  103. Bova, G. S., Eltoum, I. A., Kiernan, J. A., Siegal, G. P., Frost, A. R., Best, C. J. M., Gillespie, J. W., Su, G. H. & Emmert-Buck, M. R. 2005. Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications. Molecular Biotechnology 29, 119-152.  https:/10.1385/MB:29:2:119
  104. Kiernan, J. A. 2006. Dyes and other colorants in microtechnique and biomedical research. Coloration Technology 122, 1-21. Click here for PDF file
  105. Dapson, R. W., Frank, M. & Kiernan, J. A. 2006. Revised procedures for the certification of carmine (CI 75470, Natural red 4) as a biological stain. Biotechnic & Histochemistry  82: 13-15. https://doi.org/10.1080/10520290701207364
  106. Frank, M., Dapson, R. W., Wickersham, T. W. & Kiernan, J. A. 2007. Certification procedures for nuclear fast red (Kernechtrot), C.I. 60760. Biotechnic & Histochemistry  82: 35-39.  https://doi.org/10.1080/10520290701333475
  107. Kiernan, J. A. 2007. Histochemistry of staining methods for normal and degenerating myelin in the central and peripheral nervous systems. Journal of Histotechnology 30: 87-106. https://doi.org/10.1179/his.2007.30.2.87
  108. Kiernan, J. A. 2007. Indigogenic substrates for the detection and localization of enzymes.  Biotechnic & Histochemistry 82: 73-103. https://doi.org/10.1080/10520290701375278
  109. Kiernan, J. A. 2009. A system for quantitative evaluation of morphological effects of fixatives for light microscopy, using paraffin sections of kidney and brain. Biotechnic & Histochemistry 84: 1-10. https://doi.org/10.1080/10520290802646619
  110. Kiernan, J. A. 2009. Graduate training and education in histotechnology and histochemistry. Journal of Histotechnology 32: 115-118. https://doi.org/10.1179/his.2009.32.3.115 
  111.  Dapson, R., Horobin, R. W. & Kiernan, J. A. 2010. Hematoxylin shortages: their causes and duration, and other dyes that can replace hemalum in routine H & E staining. Biotechnic & Histochemistry 85: 55-63. https://doi.org/10.3109/10520290903048400
  112. Dapson, R. W., Fagan, C., Kiernan, J. A. & Wickersham, T. W. 2011. Certification procedures for sirius red F3B (CI 35780, Direct red 80). Biotechnic & Histochemistry 86: 133-139. https://doi.org/10.3109/10520295.2011.570277
  113. Kiernan, J. A. 2012. Anatomy of the temporal lobe. Epilepsy Research and Treatment  Vol. 2012: Article ID 176157, 12 pages. https://doi.org/10.1155/2012/176157.
  114. Owen, M. A., Schauwers, W., Hugh-Jones, M. E., Kiernan, J. A., Turnbull, P. C. B. & Beyer, W. 2013. A simple, reliable M’Fadyean stain for visualizing the Bacillus anthracis capsule. Journal of Microbiology Methods 92: 264-269. https://doi.org/10.1016/j.mimet.2013.01.009 
  115. Kiernan, J. A. 2017. Buffered ethanol fixative. (Letter)  Journal of Histochemistry and Cytochemistry 65: 251-252. https://doi.org/10.1369/0022155416687278
  116. Kiernan, J. A. 2018.  Does progressive nuclear staining with hemalum (alum hematoxylin) involve DNA, and what is the nature of the dye-chromatin complex? Biotechnic & Histochemistry  92 (2): 133-148.  https://doi.org/10.1080/10520295.2017.1399466 
  117. Dunmore-Buyze, P. J., Cruje, C., Nong, Z., Lee, J., Kiernan, J. A., Pickering, J. G. & Drangova, M. 2019. 3D vessel-wall virtual histology of whole-body perfused mice using a novel heavy element stain.  Scientific Reports 9(1): Article number 698.  https://doi.org/10.1038/s41598-018-36905-z
  118. Fagan, C., Dapson, R. W., Horobin, R. W. & Kiernan, J. A. 2020. Revised tests and standards for certification of alcian blue dyes by the Biological Stain Commission. Biotechnic & Histochemistry 95(5):  333-340.   https://doi.org/10.1080/10520295.2019.1699163
  119. Fagan, C. & Kiernan, J. A. 2020. Certification procedures used by the Biological Stain Commission for eriochrome cyanine R (C.I. 43820, Mordant blue 3). Biotechnic & Histochemistry 95(5): 396-402. https://www.tandfonline.com/doi/full/10.1080/10520295.2020.1748229
  120. Ali, S.,  Dapson, R. W.,  Kiernan, J. A., Horobin, R. W. &  Kazlaunicias, A. 2022. At least four distinct blue cationic phthalocyanine dyes are sold as “alcian blue”. So, what is alcian blue?  Biotechnic & Histochemistry  97(1): 11-22.    https://doi.org/10.1080/10520295.2021.2018497

                                   

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Books

  1. Kiernan, J. A. 1981. Histological and Histochemical Methods: Theory and Practice. Oxford: Pergamon Press.
  2. Barr, M. L. and Kiernan, J. A. 1983. The Human Nervous System. An Anatomical Viewpoint. 4th edition. Philadelphia: Harper and Row.
  3. Kiernan, J. A. 1987. An Introduction to Human Neuroscience. Philadelphia: Lippincott.
  4. Barr, M. L. and Kiernan, J. A. 1988. The Human Nervous System. An Anatomical Viewpoint. 5th edition. Philadelphia: Lippincott.
  5. Kiernan, J. A. 1990. Histological and Histochemical Methods: Theory and Practice. 2nd edition. Oxford: Pergamon Press.
  6. Barr, M. L. and Kiernan, J. A. 1993. The Human Nervous System. An Anatomical Viewpoint. 6th edition. Philadelphia: Lippincott.
  7. Kiernan, J. A. 1998. Barr's The Human Nervous System: An Anatomical Viewpoint. 7th edition. Philadelphia: Lippincott-Raven.
  8. Kiernan, J. A. 1999. Histological and Histochemical Methods: Theory and Practice. 3rd edition. Oxford: Butterworth-Heinemann. (Paperback edition published in 2000 by Arnold Publishers, London)
  9. Horobin, R. W. and Kiernan, J. A. (Eds) 2002. Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for Use in Biology and Medicine. 10th ed. Oxford: BIOS Scientific Publishers (ISBN  1859960995).
  10. Kiernan, J. A. and Mason, I. G. (Eds) 2002. Microscopy and Histology for Molecular Biologists. A User's Guide. London: Portland Press (ISBN 1855781417 hard cover, 1855781565 paperback).
  11. Kiernan, J. A. 2005. Barr's The Human Nervous System: An Anatomical Viewpoint. 8th edition. Baltimore: Lippincott, Williams & Wilkins.
  12. Kiernan, J. A. 2008. Histological and Histochemical Methods: Theory and Practice. 4th edition. Bloxham, UK: Scion Publishing. (ISBN 9781904842422)
  13. Kiernan, J. A. 2009. Barr's The Human Nervous System: An Anatomical Viewpoint. 9th edition. Baltimore: Lippincott, Williams & Wilkins (ISBN 9780781782562).
  14. Kumar, G. L. and Kiernan, J. A. (Eds) 2010. Education Guide: Special Stains and H & E. 2nd ed. Carpinteria, CA: Dako North America. 300 pages. Download from    https://www.agilent.com/cs/library/technicaloverviews/public/08066_special_stains_eduguide.pdf
  15. Kiernan, J. A. & Rajakumar, N. 2014.  Barr's The Human Nervous System: An Anatomical Viewpoint. 10th edition. Baltimore: Lippincott, Williams & Wilkins (ISBN 9781451173277).
  16. Kiernan, J. A. 2015. Histological and Histochemical Methods: Theory and Practice. 5th edition. Banbury, UK: Scion Publishing. (ISBN 9781907904325).
                                   

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Chapters in books

  1. Kiernan, J. A. & Berry, M. 1975. Neuroanatomical methods. Chapter 1 in Methods in Brain Research, ed. P. B. Bradley. London: John Wiley. pp. 1-77.
  2. Kiernan, J. A. 1984. Role of mast cells in vascular responses due to axon reflexes in injured skin. In Antidromic Vasodilatation and Neurogenic Inflammation, ed. L. A. Chahl, J. Szolczanyi and F. Lembeck. Budapest: Akademiai Kiado. pp. 223-243.
  3. Kiernan, J. A. 1992. Some functional pathways in the central nervous system. Chapter 450 in Textbook of Internal Medicine, 2nd ed., ed. W. N. Kelley, Vol. 2, pp. 2138-2145. Philadelphia: Lippincott.
  4. Kiernan, J. A. 1997. Some functional pathways in the central nervous system. Chapter 17 in Textbook of Internal Medicine, 3rd ed., ed. W. N. Kelley, pp. 94-102. Philadelphia: Lippincott-Raven.
  5. Kiernan, J. A. 2000. Some functional pathways in the central nervous system. Chapter 14 in Kelley's Textbook of Internal Medicine, 4th ed., ed. H. D. Humes, pp. 99-108. Philadelphia: Lippincott, Williams and Wilkins.
  6. Kiernan, J. A. 2002. Freezing and fixation. Chapter 8 in Microscopy and Histology for Molecular Biologists. A User's Guide, ed. Kiernan, J. A. & Mason, I. G. London: Portland Press.
  7. Kiernan, J. A. 2002. Staining and counterstaining to show structural features. Chapter 10 in Microscopy and Histology for Molecular Biologists. A User's Guide, ed. Kiernan, J. A. & Mason, I. G. London: Portland Press.
  8. Kiernan, J. A. 2002. Nomenclature and classification of dyes and other coloring agents. Chapter 3 in Horobin, R. W. & Kiernan, J. A. (eds) Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for use in Biology and Medicine. 10th ed. Oxford: BIOS. pp. 23-39.
  9. Kiernan, J. A. & Horobin, R. W. 2002. Miscellaneous inorganic and organic substances used as biological stains. Chapter 27 in Horobin, R. W. & Kiernan, J. A. (eds) Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for use in Biology and Medicine. 10th ed. Oxford: BIOS. pp. 389-415.
  10. Bova, G. S., Eltoum, I. A., Kiernan, J. A., Siegal, G. P., Frost, A. R., Best, C. J. M., Gillespie, J. W. & Emmert-Buck, M. R. 2004. Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications. Ch. 2 in Su, G. H. ed. Pancreatic Cancer. Methods and Protocols. (Methods in Molecular Medicine, Vol. 103). Totowa, NJ: Humana Press. pp. 15-66. (Also as an e-Book: http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-780-7&txtSearchStartPos=1)
  11. Carriel, V., Campos, F., Aneiros-Fernandez, J. & Kiernan J. A. 2017. Tissue fixation and processing for the histological identification of lipids. Chapter 14 in: Pellicciari C, Biggiogera M, eds. Histochemistry of Single Molecules: Methods and Protocols. (Methods in Molecular Biology, vol. 1560) New York: Springer Science+Business Media LLC. pp. 197-206. (ISBN 9781493967889)

Other publications

These include a few unrefereed abstracts of presentations at meetings and several other informal publications. The only Abstracts listed are those containing information that was not also published in peer-reviewed papers.
                                   

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  1. Kiernan, J. A. 1965. Some histochemical observations relating to the so-called ground substance of the central nervous system. Journal of Anatomy 99, 926-928.
  2. Kiernan, J. A. 1965. Application of a fluorescent periodic acid-Schiff technique to the central nervous system. Journal of Anatomy 99, 940.
  3. Kiernan, J. A. 1970. Two types of axonal regeneration in the neurohypophysis of the rat. Journal of Anatomy 107, 187.
  4. Kiernan, J. A. 1971. Subcellular studies of axonal argyrophilia. Journal of Anatomy 108, 623.
  5. Kiernan, J. A. 1996. Murray Llewellyn Barr (1908-1995). (Obituary) Journal of Anatomy 188, 747-748.
  6. Kiernan, J. A. 1997. Making and using aqueous mounting media. Microscopy Today 97-10, 16-17.
  7. Kiernan, J. A. 1998. Double embedding in cellulose nitrate and paraffin wax, an old and useful method that is often misunderstood. Microscopy Today 98-2, 12-14.
  8. Kiernan, J. A. 1999. Long-lasting aqueous mounts for light microscopy. Microscopy Today 99-1, 3.
  9. Kiernan, J. A. 1999. Strategies for preventing detachment of sections from glass slides. Microscopy Today 99-6, 22-24.
  10. Kiernan, J. A. 1999. Recycling gold. Microscopy Today 99-10, 23-25.
  11. Kiernan, J. A. 1999. Staining and microscopy: the need for old and new methods. (Guest editorial) Biotechnic and Histochemistry 74, 275.
  12. Kiernan, J. A. 2000. Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: What they are and what they do. Microscopy Today 00-1, 8-12.
  13. Kiernan, J. A. 2000. Rit scarlet No. 5 as a stain for amyloid. (Letter, commenting on paper in J. Histotechnol. 22: [1] 33-35, 1999) Journal of Histotechnology 23, 87.
  14. Kiernan, J. A. 2000. Preventing lost tissues: a look at charged slides. What are the exact differences between poly-L-lysine and aminoalkyloxysilanes? Paraffin Press (Connecticut Society of Histotechnologists) Spring 2000, 3.
  15. Kiernan, J. A. 2000. Dehydrating delicate specimens for light microscopy. Microscopy Today 00-7, 37.
  16. Kiernan, J. A. 2001. How to stain motor end plates. Microscopy Today 01-5: 29.
  17. Kiernan, J. A. 2001. How acids and chelating agents soften bones and teeth. Hard times. Communiqué‚ of the Hard Tissue Committee, National Society for Histotechnology 13 (2) July 2001. (mini-review article)
  18. Kiernan, J. A., Pyle, R. H. & Silverman, J. S. 2001. N.S.H. Self Assessment Examinations, Series 5: Immunohistochemistry, Enzyme Histochemistry, Flow Cytometry, In-Situ Hybridization, Electron Microscopy. 2nd ed. Bowie, MD, National Society for Histotechnology.
  19. Kiernan, J. A. (section editor) 2002- . Notes and Queries. A regular feature comprising peer-reviewed answers to readers' questions. Biotechnic & Histochemistry Vol. 76 onwards.
  20. Kiernan, J. A. 2005. Preservation and retrieval of antigens for immunohistochemistry - methods and mechanisms. 1. Effects of formaldehyde fixation. The Cutting Edge January 2005, pp. 5-9. (National Society for Histotechnology, Newsletter for Region IX, Canada)
  21. Kiernan, J. A. 2005. Preservation and retrieval of antigens for immunohistochemistry - methods and mechanisms. Part 2. Retrieving masked antigens. The Cutting Edge April 2005, pp. 5-11. (National Society for Histotechnology, Newsletter for Region IX, Canada)
  22. Kiernan, J. A. 2005. Nissl: the man, the stain and the substance. Microscopy Today 13(6), 50.
  23. Kiernan, J. A. & Sims, S. M. 2006. Preface,  in Hudson, A. J. (2006) The Physiological Basis of Memory and Consciousness. Lewiston, NY: Edwin Mellen Press.
  24. Lyon, H. O. & Kiernan, J. A. 2008. News from the Biological Stain Commission.  Biotechnic & Histochemistry 83: 201-203.
  25. Lyon, H. O. & Kiernan, J. A. 2008. News from the Biological Stain Commission.  Biotechnic & Histochemistry 83: 285-288.
  26. Kiernan, J. A. 2008. Nuclear Stains. Cold Spring Harbor Protocols 3:(7): doi: 10.1101/pdb.top50 (14 pages).
  27. Kiernan, J. A. 2008. Anionic counterstains. Cold Spring Harbor Protocols 3:(7): doi: 10.1101/pdb.top51 (2 pages).
  28. Kiernan, J. A. & Horobin, R. W. 2010. A special issue devoted to hematoxylin, hematein and hemalum.  Biotechnic & Histochemistry 85: 5-6. (Editorial).
  29. Kumar, G. L. & Kiernan, J. A. 2010. Editorial.  Special stains and H&E. Connection (Dako Inc. Scientific Magazine). Vol. 14, p. 14.
  30. Kiernan, J. A. 2010. Staining sections of the central nervous system. Connection (Dako Inc. Scientific Magazine). Vol. 14, pp. 60-67.
  31. Kiernan, J. A. 2010. Carbohydrate histochemistry. Connection (Dako Inc. Scientific Magazine). Vol. 14, pp. 68-78.
  32. Kiernan, J. A. 2010. On chemical reactions and staining mechanisms. Connection (Dako Inc. Scientific Magazine). Vol. 14, pp. 127-136. [What is the difference between argentaffin and argyrophilic reactiuons? (p. 127-128). How does pH influence the staining mechanism of Schiff's reagent in biological tissue samples? (p. 129-131). What is Giemsa’s stain and how does it color blood cells, bacteria and chromosomes? (p. 132-136).]
  33. Kumar, G. L. & Kiernan, J. A. Preface, in Kumar, G. L. & Kiernan, J. A. (eds) 2010. Education Guide: Special Stains and H & E. 2nd ed. Carpinteria, CA: Dako North America, p. ix.
  34. Kiernan, J. A. 2013. Editorial.  Journal of Histotechnology 36(1): 1-2.  DOI: 10.1179/0147888513Z.00000000041 (Summary and brief discussion of papers in the March 2013 issue of the journal) 
  35. Kiernan, J. A. 2014. Editorial.  Journal of Histotechnology 37(2):  41-42. DOI: 10.1179/0147888514Z.00000000085 (Summary and brief discussion of papers in the June  2014 issue of the journal) 
  36. Kiernan, J. A. 2016. Editorial: Innovations, applications and more innovations - a continuing tradition in histotechnical publication.  Journal of Histotechnology 39(2):  37-38.  DOI: 10.1080/01478885.2016.1173969  (Comments on papers in the June 2016 issue of the journal)
  37. Kiernan, J. A. 2017. Editorial. Fibers, bacteria and things that look like fungi under the microscope. Journal of Histotechnology 40(2):  29-30.
  38. Kiernan, J. A. 2018. Book review: Fluorescence Microscopy in Life Sciences, by Juan Carlos Stockert and Alfonso Blázquez-Castro. Bentham Science Publishers, Sharjah, UAE, 2017. Journal of Histotechnology 41(4): 195-196.  DOI:10.1080/014778885.2018.1514715  https://www.tandfonline.com/eprint/Z4sCX7KkdhFBh37UfsrK/full
  39. Dapson, R. W., Horobin, R. W., Kiernan, J. A. 2019. Glossary of staining methods, reagents, immunostaining, terminology and eponyms. Version 1.2.  Biological Stain Commission, Inc.  https://biologicalstaincommission.org/bscglossary.html
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Last updated: January 2024