Restriction Map of Ad5

  • restriction enzymes cutting 1-4 times, basepairs 1-1774
    Enzyme     Positions of Recognition Sites             
    AccB1I          814 1302                                        
    AccB7I          1470                                            
    AccBSI          542                                             
    AccI            1107                                          
    AccIII          826                                           
    AclWI           729                                           
    AcsI            242 1201                                        
    AcyI            815 1303 1320 1458                            
    AfaI            194 639 906 1359                              
    AflIII          143 458                                        
    Alw21I          1005 1774                                     
    Alw26I          384 566 1321                                   
    AlwI            729                                             
    Ama87I          854 1007 1255                                   
    ApoI            242 1201                                      
    AspHI           1005 1774                                       
    AvaI            854 1007 1255                                   
    BalI            270                                             
    BanI            814 1302                                        
    BanII           1256 1774                                       
    BanIII          918                                             
    BbeI            818 1306                                        
    BbiII           815 1303 1320 1458                              
    Bbv12I          1005 1774                                       
    BbvI            854 863                                       
    BcoI            854 1007 1255                                   
    BfaI            348 663 1340                                    
    Bsa29I          918                                            
    BsaAI           376                                             
    BsaHI           815 1303 1320 1458                             
    BsaMI           1350                                         
    BscI            918                                            
    Bse118I         187 811                                        
    Bse1I           613 644 955 1439                                
    BseAI           826                                             
    BseCI           918                                             
    BseNI           613 644 955 1439                               
    BseRI           532 739 982                                    
    BsgI            784                                             
    BshNI           814 1302                                        
    BsiHKAI         1005 1774                                      
    BsiI            937                                             
    BsiMI           826                                             
    BsmAI           384 566 1321                                    
    BsmBI           1322                                            
    BsmFI           363 554 1057 1418                               
    BsmI            1350                                         
    BsoBI           854 1007 1255                                   
    Bsp106I         918                                           
    Bsp13I          826                                             
    Bsp1407I        192                                            
    Bsp143I         627 724 915 919                                
    Bsp143II        818 1306                                       
    BspDI           918                                             
    BspEI           826                                            
    BspLU11I        143                                            
    BspMI           931 1025                                                  
    BspXI           918                                            
    BsrBI           542                                             
    BsrFI           187 811                                        
    BsrGI           192                                             
    BsrI            613 644 955 1439                               
    BsrSI           613 644 955 1439                                
    BssAI           187 811                                        
    BssSI           937                                             
    BssT1I          876                                           
    Bst2UI          390 1465 1502 1536                             
    Bst71I          854 863                                       
    BstD102I        542                                            
    BstDSI          354 577 1683                                    
    BstF5I          160 234 968                                   
    BstH2I          818 1306                                        
    BstNI           390 1465 1502 1536                             
    BstOI           390 1465 1502 1536                              
    BstSFI          1108                                            
    BstX2I          724                                             
    BstXI           616 938                                         
    BstYI           724                                            
    Bsu15I          918                                            
    Cfr10I          187 811                                        
    Cfr42I          357                                             
    Cfr9I           1007                                           
    CfrI            268 603                                         
    ClaI            918                                            
    Csp6I           193 638 905 1358                                
    DpnI            629 726 917 921                                 
    DpnII           627 724 915 919                                 
    DraI            1197 1223                                       
    DraII           1054 1089 1228                                  
    DraIII          953 1410                                        
    DsaI            354 577 1683                                    
    EaeI            268 603                                         
    Eam1104I        637 973                                         
    EarI            637 973                                         
    Ecl136II        1772                                            
    Eco130I         876                                             
    Eco24I          1256 1774                                       
    Eco64I          814 1302                                        
    Eco88I          854 1007 1255                                   
    EcoICRI         1772                                            
    EcoNI           1714                                            
    EcoO109I        1054 1089 1228                                  
    EcoRII          388 1463 1500 1534                              
    EcoT14I         876                                             
    EheI            816 1304                                       
    ErhI            876                                             
    Esp1396I        1470                                            
    Esp3I           1322                                            
    FokI            160 234 968                                     
    FriOI           1256 1774                                      
    HaeII           818 1306                                        
    HgaI            1323 1462                                      
    Hin1I           815 1303 1320 1458                             
    HincII          1574             
    HindII          1574                                            
    HinfI           382 761 1372                                   
    HpaI            1574                                            
    Hsp92I          815 1303 1320 1458                             
    Hsp92II         147 1102 1642 1716                              
    KasI            814 1302                                        
    Kpn2I           826                                             
    Ksp632I         637 973                                         
    KspI            357                                             
    Kzo9I           627 724 915 919                                 
    MaeI            348 663 1340                                   
    MboI            627 724 915 919                                 
    MflI            724                                             
    MluNI           270                                             
    MroI            826                                             
    MroNI           811                                             
    MscI            270                                             
    MslI            582                                            
    Msp17I          815 1303 1320 1458                              
    MspA1I          356 454 625 1419                               
    Mva1269I        1350                                            
    MvaI            390 1465 1502 1536                              
    NaeI            813                                             
    NarI            815 1303                                        
    NdeII           627 724 915 919                                 
    NgoAIV          811                                             
    NgoMI           811                                            
    NlaIII          147 1102 1642 1716                              
    NspBII          356 454 625 1419                                
    NspI            147 1102                                        
    PflMI           1470                                            
    PleI            386 765 1376                                    
    PpuMI           1054 1089 1228                                  
    PshAI           1376                                            
    Psp124BI        1774                                            
    Psp5II          1054 1089 1228                                  
    PspAI           1007                                            
    PspALI          1009                                            
    PvuII           454 625                                         
    RsaI            194 639 906 1359                                
    SacI            1774                                            
    SacII           357                                             
    Sau3AI          627 724 915 919                                 
    SfcI            1108                                            
    Sfr303I         357                                             
    SgrAI           187 811                                         
    SmaI            1009                                            
    SspBI           192                                             
    SspI            341                                             
    SstI            1774                                           
    SstII           357                                             
    StyI            876                                             
    TaqI            630 918 1481                                  
    TspRI           956 1135                                        
    TthHB8I         630 918 1481                                   
    Van91I          1470                                            
    XbaI            1339                                            
    XhoII           724                                            
    XmaI            1007                                          
    
    This analysis was generated by Webcutter, copyright 1995 Max Heiman, at http://firstmarket.com/firstmarket/cutter and http://www.medkem.gu.se/cutter
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