UWO Physics and Astronomy BuildingPublications of the Konermann Laboratorymyoglobin

 

 

Konermann Home

Lars Konermann

Konermann Research

Konermann People

Konermann Publications

Konermann News

2018 | 2017 | 2016

2015 | 2014 | 2013 | 2012 | 2011 | 2010

2009 | 2008 | 2007 | 2006 | 2005 | 2004

2003 | 2002 | 2001 | 2000 | 1999 | earlier work


2017

"Cytochrome c as a Peroxidase: Activation of the Pre-Catalytic Native State by H2O2-Induced Covalent Modifications" V. Yin, G. S. Shaw, and L. Konermann J. Am. Chem. Soc. 139, 15701-15709 (2017). Online access.

 

"Calcium-Mediated Control of S100 Proteins: Allosteric Communication via an Agitator/Signal Blocking Mechanism" Y. Xiao, G. S. Shaw, and L. Konermann J. Am. Chem. Soc. 139, 11460-11470 (2017). Online access.
See also JACS Spotlight J. Am. Chem Soc. 139, 12103-12104 (2017).

 

"Changes in Enzyme Structural Dynamics Studied by Hydrogen Exchange-Mass Spectrometry: Ligand Binding Effects or Catalytically Relevant Motions?" C. S. Fast, S. Vahidi, and L. Konermann Anal. Chem. in press (2017). Online access.

 

"Molecular Dynamics Simulations on Gas Phase Proteins with Mobile Protons: Inclusion of All-Atom Charge Solvation" L. Konermann J. Phys. Chem. B 121, 8102-8112 (2017). Online access.

 

"Addressing A Common Misconception: Ammonium Acetate as Neutral pH “Buffer” for Native Electrospray Mass Spectrometry" L. Konermann J. Am. Soc. Mass Spectrom. 28, 1827-1835 (2017). Online access.

JASMS TOC Figure

 

"Characterizing the Structure and Oligomerization of Major Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and Complementary Biophysical Tools" S. C. Mandacaru, L. H. F. do Vale, S. Vahidi, Y. Xiao, O. S. Skinner, C. A. O. Ricart, N. L. Kelleher, M. V. de Sousa, and L. Konermann Biochemistry 56, 1645–1655 (2017). Online access.

 

TOC Biochemistry 2017a

 

back to top



2016

 

"Load-dependent destabilization of the gamma-rotor shaft in FOF1 ATP synthase revealed by H/D-exchange mass spectrometry" S. Vahidi, Y. Bi, S. D. Dunn, and L. Konermann Proc. Natl. Acad. Sci. U.S.A. 113, 2412-2417 (2016). Online access.

 

"Heavy lessons in protein allostery" L. Konermann Nat. Struct. Mol. Biol. 23, 511-512 (2016). Online access.

 

"Conformational Characterization of the Intrinsically Disordered Protein Chibby: Interplay Between Structural Elements in Target Recognition" R. C. Killoran, M. A. Sowole, M. A. Halim, L. Konermann, and W.-Y. Choy Protein Sci. 25, 1420-1429 (2016). Online access.

 

"Evidence for Allosteric Enzyme Regulation via Changes in Conformational Dynamics: An H/D Exchange Investigation of Dihydrodipicolinate Synthase" M. A. Sowole, S. Simpson, Y. V. Skovpen, D. R. J. Palmer, and L. Konermann Biochemistry 55, 5413-5422 (2016). Online access.

 

"Collision-Induced Dissociation of Electrosprayed NaCl Clusters: Using Molecular Dynamics Simulations to Visualize Reaction Cascades in the Gas Phase" T. D. Schachel, H. Metwally, V. Popa, and L. Konermann J. Am. Soc. Mass Spectrom. 27, 1846-1854 (2016). Online access.

 

"Effects of Multidentate Metal Interactions on the Structure of Collisionally Activated Proteins: Insights from Ion Mobility Spectrometry and Molecular Dynamics Simulations" C. E. Bartman, H. Metwally, and L. Konermann Anal. Chem. 88, 6905-6913 (2016). Online access.

 

"Collision-Induced Dissociation of Electrosprayed Protein Complexes: An All-Atom Molecular Dynamics Model with Mobile Protons" V. Popa, D. Trecroce, R. G. McAllister, and L. Konermann J. Phys. Chem. B, 120 5114−5124 (2016). Online access.

 

"Mechanism of Protein Supercharging by Sulfolane and m-NBA: Molecular Dynamics Simulations of the Electrospray Process" H. Metwally, R. G. McAllister, V. Popa, and L. Konermann Anal. Chem. 88, 5345-5354 (2016). Online access.

 

 

"Probing the Time Scale of FPOP (Fast Photochemical Oxidation of Proteins): Radical Reactions Extend Over Tens of Milliseconds" S. Vahidi and L. Konermann J. Am. Soc. Mass Spectrom. 27, 1156-1164 (2016). Online access.

 

"Protein Structural Studies by Traveling Wave Ion Mobility Spectrometry: A Critical Look at Electrospray Sources and Calibration Issues" Y. Sun, S. Vahidi, M. A. Sowole, and L. Konermann J. Am. Soc. Mass Spectrom. 27, 31-40 (2016). Online access.

back to top



2015

"Release of Native-Like Gaseous Proteins from Electrospray Droplets via The Charged Residue Mechanism: Insights from Molecular Dynamics Simulations" R. G. McAllister, H. Metwally, Y. Sun, and L. Konermann J. Am. Chem. Soc. 137, 12667-12676 (2015). Online access. See also JACS Spotlight, J. Am. Chem. Soc. 137, 11855 (2015).

Supporting Movie Examples: ubiquitin in water, ubiquitin in water with NaCl

 

"Interactions of Hemoglobin and Myoglobin with their Ligands CN-, CO, and O2 Monitored by Electrospray Mass Spectrometry" M. A. Sowole, S. Vuong, and L. Konermann Anal. Chem. 87, 9538–9545 (2015). Editor's Highlight.
Online access
.

 

"Challenges in the Interpretation of Protein H/D Exchange Data: A Molecular Dynamics Simulation Perspective" R. G. McAllister and L. Konermann Biochemistry 54, 2683-2692 (2015). Online access.


"Exploring the Mechanism of Salt-Induced Signal Suppression in Protein Electrospray Mass Spectrometry Using Experiments and Molecular Dynamics Simulations", H. Metwally, R. G. McAllister, and L. Konermann Anal. Chem. 87, 2434-2442 (2015). Online access.

 

"Noncovalent Binding of a Cyclic Peptide Inhibitor to the Peptidyl-Prolyl Isomerase Pin1 Explored by Hydrogen Exchange Mass Spectrometry" M. A. Sowole, B. T. Innes, M. Amunugama, D. W. Litchfield, C. J. Brandl, B. H. Shilton, and L. Konermann Can. J. Chem. 93, 44-50 (2015). Online access.

 

"Peroxide-Mediated Oxidation and Inhibition of the Peptidyl-Prolyl Isomerase Pin1" B. T. Innes, M. A Sowole, L. Gyenis, M. Dubinsky, L. Konermann, D. W. Litchfield, C. J. Brandl, and B. H. Shilton Biochim. Biophys. Acta 1852, 905-912 (2015). Online access.

 

"Protein Structural Dynamics at the Gas/Water Interface Examined by Hydrogen Exchange Mass Spectrometry" Y. Xiao and L. Konermann Protein Sci. 24, 1247-1256 (2015). Online access.

back to top



2014

"Type 1 and Type 2 Scenarios in Hydrogen Exchange Mass Spectrometry Studies on Protein-Ligand Complexes" L. Konermann, A. D. Rodriguez, M. A. Sowole Analyst 139, 6078-6087 (2014). Online access.

 

"Molecular Dynamics Simulations of the Electrospray Process: Formation of NaCl Clusters via the Charged Residue Mechanism" L. Konermann, R. G. McAllister, H. Metwally J. Phys. Chem. B 118, 12025–12033 (2014). Online access.

Supporting Movies: early droplet, late droplet, dry salt cluster

TOC JPCB 2014

 

"Effects of Protein-Ligand Interactions on Hydrogen/Deuterium Exchange Kinetics: Canonical and Non-Canonical Scenarios" M. A. Sowole and L. Konermann Anal. Chem. 86, 6715-6722 (2014). Online access.

Anal Chem TOC 2014

 

"ATP-Induced Dimerization of the FOF1 Epsilon Subunit from Bacillus PS3: A Hydrogen Exchange/Mass Spectrometry Study " A. D. Rodriguez, S. D. Dunn, and L. Konermann Biochemistry 53, 4072-4080 (2014). Online access.

Biochemistry TOC

 

"Insights Into the Mechanism of Protein Electrospray Ionization From Salt Adduction Measurements" X. Yue, S. Vahidi, and L. Konermann J. Am. Soc. Mass Spectrom. 25, 1322-1331 (2014). Online access.

JASMS TOC

 

"Mass Spectrometry Methods for Studying Structure and Dynamics of Biological Macromolecules" L. Konermann, S. Vahidi, M. A. Sowole Anal. Chem. 86, 213-232 (2014). Online access.

 

"Cation-Induced Stabilization of Protein Complexes in the Gas Phase: Mechanistic Insights From Hemoglobin Dissociation Studies" J. Liu and L. Konermann J. Am. Soc. Mass Spectrom. 25, 595-603 (2014). Online access.


back to top


 


2013

"Submillisecond Protein Folding Events Monitored by Rapid Mixing and Mass Spectrometry-Based Oxidative Labeling" S. Vahidi, B. B. Stocks, Y. Liaghati-Mobarhan, and L. Konermann Anal. Chem. 85, 8618–8625 (2013). Online acess. Cover feature.

 

 

 

TOC figure

Graphical abstract.

AC cover
Click on image for larger version of journal cover.

 

"Unraveling The Mechanism of Electrospray Ionization" L. Konermann, E. Ahadi, A. D. Rodriguez, and S. Vahidi Anal. Chem. 85, 2-9 (2013). Online access. Cover feature, 4th most read 2013 Anal. Chem. paper.
Author interview podcast (.mp3).



Graphical abstract.

Click on image for larger version of journal cover.

 

"Activation of ClpP Protease by ADEP Antibiotics: Insights from Hydrogen Exchange Mass Spectrometry" M. Sowole,  J. Alexopoulos, Y.-Q.  Cheng, J. Ortega, and L. Konermann J. Mol. Biol. 425 4508-4519 (2013). Online access.

  TOC figure  


"Effects of Ammonium Bicarbonate on the Electrospray Mass Spectra of Proteins: Evidence for Bubble-Induced Unfolding" J. B. Hedges, S. Vahidi, X. Yue, and L. Konermann Anal. Chem. 85, 6469-6476 (2013). Online acess.



 

"Nanocluster Isotope Distributions Measured by Electrospray Time-of-Flight Mass Spectrometry" A.N. Comeau, J. Liu, C. B. Khadka, J. F. Corrigan, and L. Konermann Anal. Chem. 85, 1200-1207 (2013). Online access.




"Assembly of Hemoglobin from Denatured Monomeric Subunits: Heme Ligation Effects and Off-Pathway Intermediates Studied by Electrospray Mass Spectrometry" J. Liu and L. Konermann Biochemistry 52, 1717-1724 (2013).
Online access.



"Partially Disordered Proteins Studied by Ion Mobility-Mass Spectrometry: Implications for the Preservation of Solution Phase Structure in the Gas Phase" S. Vahidi, B. B. Stocks, and L.Konermann Anal. Chem. 85 10471-10478 (2013). Online access.

TOC figure

 

"Comparative Analysis of Oxy-Hemoglobin and Aquomet-Hemoglobin by Hydrogen/Deuterium Exchange Mass Spectrometry" M. A. Sowole and L. Konermann J. Am. Soc. Mass Spectrom. 24, 997-1005 (2013). Online access.


back to top



2012

"Mapping pH-Induced Protein Structural Changes Under Equilibrium Conditions by Pulsed Oxidative Labeling and Mass Spectrometry" S. Vahidi, B. B. Stocks, Y. Liaghati-Mobarhan, and L. Konermann Anal. Chem. 84, 9124–9130 (2012). Online access.



"Early Hydrophobic Collapse of a1-Antitrypsin Facilitates Formation of a Metastable State: Insights from Oxidative Labeling and Mass Spectrometry" B. B. Stocks, A. Sarkar, P. L. Wintrode, and L. Konermann J. Mol. Biol. 423, 789-799  (2012). Online access.



"On the Formation of Highly Charged Gaseous Ions from Unfolded Proteins by Electrospray Ionization" L. Konermann, A. D. Rodriguez, J. Liu Anal. Chem. 84, 6798–6804 (2012). Online access.



"Structure and Dynamics of Small Soluble Abeta(1-40) Oligomers Studied by Top-Down Hydrogen Exchange Mass Spectrometry" J. Pan, J. Han, C. H. Borchers, and L. Konermann Biochemistry 51, 3694–3703 (2012). Online access.




"Conformational Dynamics of a Membrane Transport Protein Probed by H/D Exchange and Covalent Labeling: The Glycerol Facilitator"  Y. Pan, H. Piyadasa, J. D. O'Neil, and L. Konermann J. Mol. Biol. 416, 400-413 (2012). Online access. Highlighted in Chemical and Engineering News 90,  11-15 (2012).


This work was also featured on the cover of the Anal. Chem. March 5, 2013 issue, for a review by J. Whitelegge.



"Modeling the Behavior of Coarse-Grained Polymer Chains in Charged Water Droplets: Implications for the Mechanism of Electrospray Ionization" Elias Ahadi and Lars Konermann J. Phys. Chem. B 116, 104–112 (2012). Online access.
Supporting Information (MPEG video):
hydrophobic/folded polymer chain, hydrophobic/unfolded polymer chain
hydrophilic/folded polymer chain, hydrophilic/unfolded polymer chain



 

"Structural Interrogation of Electrosprayed Peptide Ions by Gas-Phase H/D Exchange and Electron Capture Dissociation Mass Spectrometry" J. Pan, B. L. Heath, R. A. Jockusch, and L. Konermann Anal. Chem. 84, 373−378 (2012). Online access.



 

"Validation of Membrane Protein Topology Models by Oxidative Labeling and Mass Spectrometry" Y. Pan, X. Ruan, M. A. Valvano, and L. Konermann J. Am. Soc. Mass Spectrom. 23, 889-898 (2012). Online access.

"Correction of both NBD1 energetics and domain interface is required to restore Delta F508 CFTR folding and function" W. M. Rabeh, F. Bossard, H. Xu, T. Okiyoneda, M. Bagdany, C. M. Mulvihill, K. Du, S. di Bernardo, Y. Liu, L. Konermann, A. Roldan, G. L. Lukacs Cell
148, 150-163 (2012). Online access.

"Protein Unfolding and Denaturants" (version 2.0) L. Konermann Encyclopedia of Life Sciences, Wiley & Sons (Chichester, UK) DOI: 10.1002/9780470015902.a0003004.pub2 (2012). Online access.

"Pulsed Hydrogen/Deuterium Exchange Mass Spectrometry for Time-Resolved Membrane Protein Folding Studies" A. Khanal, Y. Pan, L. S. Brown, and L. Konermann J. Mass Spectrom. 47, 1620-1626  (2012). Online access.

"Exploring membrane protein structural features by oxidative labeling and mass spectrometry" L. Konermann and Y. Pan Exp. Rev. Proteomics
9, 497-504 (2012). Online access.


back to top




2011


"Hydrogen Exchange Mass Spectrometry of Bacteriorhodopsin Reveals Light-Induced Changes in the Structural Dynamics of a Biomolecular Machine" Y. Pan, L. Brown, and L. Konermann J. Am. Chem. Soc. 133, 20237–20244 (2011). Online access.




"Ejection of Solvated Ions from Electrosprayed Methanol/Water Nanodroplets Studied by Molecular Dynamics Simulations" E. Ahadi and L. Konermann J. Am. Chem. Soc. 133, 9354–9363 (2011). Online access.
Supporting Information (MPEG video):
water droplet, water/methanol droplet, methanol droplet




 

"Conformer-Specific Hydrogen Exchange Analysis of Abeta(1-42) Oligomers by Top-Down Electron Capture Dissociation Mass Spectrometry" J. Pan, J. Han, C. H. Borchers, and L. Konermann Anal. Chem., 83, 5386–5393 (2011). Online Access.




 

"Kinetic Folding Mechanism of an Integral Membrane Protein Examined by Pulsed Oxidative Labeling and Mass Spectrometry"  Y. Pan, L. Brown; L. Konermann J. Mol. Biol. 410, 146-158 (2011). Online access.




"Temporal Development of Protein Structure During S100A11 Folding and Dimerization Probed by Oxidative Labeling and Mass Spectrometry" B. B. Stocks, A. Rezvanpour, G. S. Shaw, and L. Konermann J. Mol. Biol. 409, 669-679 (2011). Online access.



 

"Hydrogen Exchange Mass Spectrometry for Studying Protein Structure and Dynamics" L. Konermann, J. Pan, and Y.-H. Liu Chem. Soc. Rev. 40, 1224-1234 (2011). Online access.




 

"Hydrogen/Deuterium Exchange Mass Spectrometry and Optical Spectroscopy as Complementary Tools for Studying the Structure and Dynamics of a Membrane Protein" Y. Pan, L. Brown, and L. Konermann Int. J. Mass Spectrom. 302, 3-11 (2011) [H/D Exchange Special Issue]. Online access.




"An Electrostatic Charge Partitioning Model for the Dissociation of Protein Complexes in the Gas Phase" S. V. Sciuto, J. Liu, and L. Konermann, J. Am. Soc. Mass Spectrom. 22 1679-1689 (2011). Online access.

"Protein Folding Mechanisms Studied by Pulsed Oxidative Labeling and Mass Spectrometry" L. Konermann, Y. Pan, and B. B. Stocks Curr. Op. Struct. Biol. 21, 634-640 (2011). Online access.

"Probing the Metal Composition of Ternary 12-12´-16 Nanoclusters via Electrospray Ionization Mass Spectrometry" A. Howarth, J. Liu, L. Konermann, and J. F. Corrigan Z. Anorg. Allg. Chem. 637, 1203-1206 (2011). Online access.

"Protein-Protein Binding Affinities In Solution Determined by Electrospray Mass Spectrometry" J. Liu and L. Konermann J. Am. Soc. Mass Spectrom. 22, 408-417 (2011). Online access.


back to top



2010

"Surface Charge of Electrosprayed Water Nanodroplets: A Molecular Dynamics Study" E. Ahadi and L. Konermann J. Am. Chem. Soc. 132, 11270-11277 (2010). Online access.



"Site-Directed Mutagenesis Combined with Oxidative Methionine Labeling for Probing Structural Transitions of a Membrane Protein by Mass Spectrometry" Y. Pan, L. Brown, and L. Konermann J. Am. Soc. Mass Spectrom. 21, 1947-1956 (2010). Online access.




"Calcium-Induced Structural Transitions of the Calmodulin-Melittin System Studied by Electrospray Mass Spectrometry: Conformational Sub-Populations and Metal-Unsaturated Intermediates" J. Pan and L. Konermann Biochemistry 49, 3477–3486 (2010). Online access.



"Membrane Protein Structural Insights from Chemical Labeling and Mass Spectrometry" Y. Pan and L. Konermann, Analyst 135, 1191-1200 (2010). Online access.



"Laminar Flow Effects During Laser-Induced Oxidative Labeling For Protein Structural Studies by Mass Spectrometry" L. Konermann, B. B. Stocks, T. Czarny Anal. Chem. 82, 6667–6674 (2010). Online access.


"Characterizing Short-Lived Protein Folding Intermediates by Top-Down Hydrogen Exchange Mass Spectrometry" J. Pan, J. Han, C. H. Borchers, and L. Konermann Anal. Chem., 82 8591-8597 (2010). Online access.

 

"Mass Spectrometry Combined with Oxidative Labeling for Exploring Protein Structure and Folding"  L. Konermann, B. B. Stocks, Y. Pan, and X. Tong Mass Spectrom. Rev. 29  651-667 (2010). Online access.

 

"Time-Dependent Changes in Side Chain Solvent Accessibility During Cytochrome c Folding Probed By Pulsed Oxidative Labeling and Mass Spectrometry" B. B. Stocks and L. Konermann J. Mol. Biol. 398, 362-373 (2010). Online access.


back to top



2009

"Hydrogen/Deuterium Exchange Mass Spectrometry with Top-Down Electron Capture Dissociation for Characterizing Structural Transitions of a 17 kDa Protein" J. Pan, J. Han, C. H. Borchers, and L. Konermann J. Am. Chem. Soc. 131, 12801–12808 (2009). Online access.

TOC Figure JACS 2009



"Phosphorylation-Induced Activation of the Response Regulator VraR from Staphylococcus aureus: Insights from Hydrogen Exchange Mass Spectrometry" Y.-H. Liu, A. Belcheva, L. Konermann*, and D. Golemi-Kotra* J. Mol. Biol. 391, 149-163 (2009). Online access. Cover feature. Click on image for larger version.




"Irreversible Thermal Denaturation of Cytochrome c Studied by Electrospray Mass Spectrometry" J. Liu and L. Konermann J. Am. Soc. Mass Spectrom. 20, 819-828 (2009). Online access.
TOC Fig JASMS


"Formation of Monomeric S100B and S100A11 Proteins at Low Ionic Strength" N. Marlatt, B. L. Boys, L. Konermann, and G. S. Shaw Biochemistry, 48 1954–1963 (2009). Online access.
Biochemistry TOC Figure


"A Simple Model for the Disintegration of Highly Charged Solvent Droplets during Electrospray Ionization" L. Konermann J. Am. Soc. Mass Spectrom. 20, 496-506 (2009). Online access.
TOC Fig JASMS


"Structural Characterization of an Integral Membrane Protein in its Natural Lipid Environment by Oxidative Methionine Labeling and Mass Spectrometry" Y. Pan, B. B. Stocks, L. Brown, and L. Konermann Anal. Chem. 81, 28-35 (2009). Online access.

 

"Structural Characterization of Short-Lived Protein Unfolding Intermediates by Laser-Induced Oxidative Labeling and Mass Spectrometry" B. B. Stocks and L. Konermann Anal. Chem. 81, 20-27 (2009). Online access.

 

"Mapping the Structure of an Integral Membrane Protein under Semi-Denaturing Conditions by Laser-Induced Oxidative Labeling and Mass Spectrometry" Y. Pan, L. Brown, and L. Konermann J. Mol. Biol. 394, 968-981 (2009). Online access.

 

"Solution-Phase Chelators for Suppressing Nonspecific Protein-Metal Interactions in Electrospray Mass Spectrometry" J. Pan, K. Xu, X. Yang, W.-Y. Choy, and L. Konermann Anal. Chem. 81, 5008-5015 (2009). Online access.

 

"Protein Oxidative Modifications During Electrospray Ionization: Solution Phase Electrochemistry or Corona Discharge-Induced Radical Attack?" B. L. Boys, M. C. Kuprowski, J. J. Noël, and L. Konermann Anal. Chem. 81, 4027-4034 (2009). Online access.

 

"Molecular Dynamics Simulations of Electrosprayed Water Nanodroplets: Internal Potential Gradients, Location of Excess Charge Centers, and 'Hopping' Protons" E. Ahadi and L. Konermann J. Phys. Chem. B 113, 7071-7080 (2009). Online access.

 

"Protein Folding and Protein-Ligand Interactions Monitored by Electrospray Mass Spectrometry“ P. L. Ferguson, M. C. Kuprowski, B. L. Boys, D. J. Wilson, J. Pan, and L. Konermann, Curr. Anal. Chem. 5, 186-204 (2009). Online access.

 

"Biochemical Reaction Kinetics Studied by Time-Resolved Electrospray Ionization Mass Spectrometry" L. Konermann, J. Pan, and D. J. Wilson, in Mass Spectrometry Analysis of Proteins, Editor: J. Whitelegge. Series: Comprehensive Analytical Chemistry, Vol. 52;  Elsevier, Amsterdam, The Netherlands, Chapter 5, 103-126 (2009). More information.


back to top



2008

"Electron Capture Dissociation of Electrosprayed Protein Ions for Spatially-Resolved Hydrogen Exchange Measurements" J. Pan, J. Han, C. H. Borchers, and L. Konermann J. Am. Chem. Soc. 130, 11574-11575 (2008). Online access.

JACS TOC Figure

 

"Protein structure and dynamics studied by mass spectrometry: H/D exchange, hydroxyl radical labeling, and related approaches" L. Konermann, X. Tong, and Y. Pan J. Mass Spectrom. 43, 1021-1036 (2008). Cover feature. Click on image for larger version. Online access.

JMS cover

 

"Conformational Dynamics of Free and Catalytically Active Thermolysin Are Indistinguishable by H/D Exchange Mass Spectrometry" Y.-H. Liu and L. Konermann, Biochemistry 47, 6342-6351 (2008). Online access.

TOC Fig

 

"Non-Uniform Isotope Patterns Produced by Collision-Induced Dissociation of Homogeneously Labeled Ubiquitin: Implications for Spatially-Resolved H/D Exchange ESI-MS Studies“ P. L. Ferguson and L. Konermann, Anal. Chem. 80, 4078-4086 (2008). Online access.

 

"Gamma-Ray-Mediated Oxidative Labeling for Detecting Protein Conformational Changes by Electrospray Mass Spectrometry" X. Tong, J. C. Wren, and L. Konermann, Anal. Chem. 80, 2222-2231 (2008). Online access.

 

"Mass Spectrometry-Based Methods for Studying Kinetics and Dynamics in Biological Systems" L. Konermann, J. Messinger, and W. Hillier, Biophysical Techniques in Photosynthesis, Vol. 2 (T.J. Aartsma and J. Matysik, eds.) Series: Advances in Photosynthesis and Respiration. Springer, Dordrecht, 167-190 (2008). More information.

 

"Time-Resolved Electrospray Mass Spectrometry" L. Konermann, D. J. Wilson, and D. A. Simmons, Protein Structures: Methods in Protein Structure and Stability Analysis, Editors: V. N. Uversky and E. A. Permyakov, Nova Science Publishers, Inc., New York. Chapter 2.1, 95-119 (2008). More information.


back to top



2007

"Diversity of Penicillin-Binding Proteins: Resistance Factor FmtA of Staphylococcus Aureus" X. Fan, Y.-H. Liu, D. Smith, L. Konermann, K.W. M. Siu and D. Golemi-Kotra J. Biol. Chem. 282, 35143-35152 (2007). Online access.

 

"Effects of Zinc Binding on the Structure and Dynamics of the Intrinsically Disordered Protein Prothymosin: Evidence for Metalation as an Entropic Switch" S. Yi, B. L. Boys, A. Brickenden, L. Konermann, and W.-Y. Choy Biochemistry 46 13120-13130 (2007). Online access.

Biochemistry TOC Figure

 

"Symmetric Behavior of Hemoglobin α and β Subunits During Acid-Induced Denaturation Observed by Electrospray Mass Spectrometry" B. L. Boys, M. C. Kuprowski, and L. Konermann Biochemistry 46, 10675 -10684 (2007). Online access.

TOC figure

 

"Effects of Protein Concentration on the Extent of Gamma-Ray-Mediated Oxidative Labeling Studied by Electrospray Mass Spectrometry" X. Tong, J. C. Wren, and L. Konermann, Anal. Chem. 79, 6376-6382 (2007). Online access.

 

"Analysis of Protein Mixtures by Electrospray Mass Spectrometry: Effects of Conformation and Desolvation Behavior on the Signal Intensities of Hemoglobin Subunits" M. C. Kuprowski, B. L. Boys, and L. Konermann, J. Am. Soc. Mass Spectrom.18, 1279-1285 (2007). Online access.

 

JASMS cover


"A Minimalist Model for Exploring Conformational Effects on the Electrospray Charge State Distribution of Proteins" L. Konermann J. Phys. Chem. B 111, 6534-6543 (2007). Online access.

 

"Signal Response of Co-Existing Protein Conformers in Electrospray Mass Spectrometry" M. C. Kuprowski and L. Konermann, Anal. Chem. 79, 2499-2506 (2007). Online access.

 

"A Temperature-Jump Stopped-Flow System for Monitoring Chemical Kinetics Triggered by Rapid Cooling" B. L. Boys and L. Konermann, Talanta 71 1276-1281 (2007). Online access.

 

"Folding and Assembly of Hemoglobin Monitored by Electrospray Mass Spectrometry Using an On-line Dialysis System" B. L. Boys and L. Konermann, J. Am. Soc. Mass Spectrom. 18, 8-16 (2007). Cover feature. Click on image for larger version. Online access, download statistics.

JASMS cover

 

"Hydrogen/Deuterium Scrambling During Quadrupole-Time-of-Flight MS/MS Analysis of a Zinc-Binding Protein Domain" P. L. Ferguson, J. Pan, D. J. Wilson, B. Dempsey, G. Lajoie, B. Shilton, and L. Konermann, Anal. Chem. 79, 153-160 (2007). Online access.

 

"Time-Resolved ESI-MS" L. Konermann, J. Pan, D. A. Simmons, and D. J. Wilson The Encyclopedia of Mass Spectrometry, Vol. 6 (Editors: M. L. Gross and R. M. Caprioli), Elsevier, Amsterdam 802-810 (2007). More information.



back to top

 



2006

"Enzyme conformational dynamics during catalysis and in the 'resting state' monitored by hydrogen/deuterium exchange mass spectrometry" Y.-H. Liu and L. Konermann, FEBS Lett. 580  5137-5142 (2006). Online access.

 

"Exploring the Relationship Between Funneled Energy Landscapes and Two-State Protein Folding" L. Konermann Proteins: Structure, Function, and Bioinformatics 65, 153-163 (2006). Online access.

 

"Folding Kinetics of the S100A11 Protein Dimer Studied by Time-Resolved Electrospray Mass Spectrometry and Pulsed Hydrogen/Deuterium Exchange" J. Pan, A. C. Rintala-Dempsey, Y. Li, G. S. Shaw, and L. Konermann Biochemistry 45, 3005-3013  (2006). Featured as April 2006 Hot ArticleOnline access.

BIOCHEMISTRY TOC Figure


"
Pulsed Hydrogen/Deuterium Exchange MS/MS for Studying the Relationship between Noncovalent Protein Complexes in Solution and in the Gas-Phase after Electrospray Ionization" B. M. Hossain and L. Konermann Anal. Chem. 78, 1613-1619 (2006). Online access.

"Protein Folding Mechanisms Studied by Time-Resolved Electrospray Mass Spectrometry" L. Konermann, J. Pan, and D. J. Wilson Biotechniques 40, 135-141 (2006). Online access.



back to top



2005

"Ultrarapid Desalting of Protein Solutions for Electrospray Mass Spectrometry in a Microchannel Laminar Flow Device" D. J. Wilson and L. Konermann Anal. Chem. 77, 6887-6894 (2005). Online access.

"Capillary Mixer With Adjustable Reaction Chamber Volume for Mass Spectrometry" L. Konermann and D. J. Wilson United States Patent 06870154, issued March 22, 2005.

"Mass spectrometry-based approaches to protein-ligand interactions" S. M. Schermann, D. A. Simmons, and L. Konermann Exp. Rev. Proteomics 2, 475-485 (2005). Online access.

"Pulsed Hydrogen Exchange and Electrospray Charge-State Distribution as Complementary Probes of Protein Structure in Kinetic Experiments: Implications for Ubiquitin Folding" J. Pan, D. J. Wilson, and L. Konermann Biochemistry 44, 8627-8633 (2005). Online access.

Biochemistry TOC Figure



"Do Electrospray Mass Spectra Reflect the Ligand Binding State of Proteins in Solution? " B. M. Hossain, D. A. Simmons, and L. Konermann, Can. J. Chem. (Special Issue: Dedicated to Professor P. Kebarle) 83, 1953-1960 (2005). Online access.
Can J Chem Table of Contents Figure


"Kinetic Unfolding Mechanism of the Inducible Nitric Oxide Synthase Oxygenase Domain Determined by Time-Resolved Electrospray Mass Spectrometry" D. J. Wilson, S. P. Rafferty, and L. Konermann, Biochemistry 44, 2276 - 2283 (2005). Online access.

BIOCHEMISTRY Table of Contents Figure


"The reconstitution of unfolded myoglobin with hemin dicyanide is not accelerated by fly-casting" M. O. Crespin, B. L. Boys and L. Konermann, FEBS Lett. 579, 271-274 (2005). Online access.

back to top



2004

"Subunit Disassembly and Unfolding Kinetics of Hemoglobin Studied by Time-Resolved Electrospray Mass Spectrometry" D. A. Simmons, D. J. Wilson, G. A. Lajoie, A. Doherty-Kirby, and L. Konermann, Biochemistry 43, 14792-14801 (2004). Online access.

BIOCHEMISTRY Table of Contents Figure

"2003 Fred Beamish Award Lecture - Exploring the Dynamics of Biological Systems by Mass Spectrometry" L. Konermann, Can. J. Chem. 82, 1565-1580 (2004). Cover feature. Click on image for larger version. Online access.

Can. J. Chem. cover

"Effects of Ground Loop Currents on Signal Intensities in Electrospray Mass Spectrometry" R. A. Ochran and L. Konermann, J. Am. Soc. Mass Spectrom. 15, 1748-1754 (2004). Online access.

"Determination of Ligand-Protein Dissociation Constants by Electrospray Mass Spectrometry-Based Diffusion Measurements" S. M. Clark and L. Konermann, Anal. Chem. 76, 7077-7083 (2004). Online access.

"Mechanistic Studies on Enzymatic Reactions by ESI-MS Using a Capillary Mixer With Adjustable Reaction Chamber Volume for Time-Resolved Measurements" D. J. Wilson and L. Konermann, Anal. Chem. 76, 2537-2543 (2004). Online access.

"Screening for Noncovalent Ligand-Receptor Interactions by Electrospray Ionization Mass Spectrometry-Based Diffusion Measurements" S. M. Clark and L. Konermann, Anal. Chem. 76, 1257-1263 (2004). Online access.

back to top



2003

"A Capillary Mixer With Adjustable Reaction Chamber Volume for Millisecond Time-Resolved Studies by Electrospray Mass Spectrometry" D. J. Wilson and L. Konermann, Anal. Chem. 75, 6408-6414 (2003). Online access.

"Conformational Dynamics of Partially Denatured Myoglobin Studied by Time-Resolved Electrospray Mass Spectrometry With Online Hydrogen-Deuterium Exchange" D. A. Simmons, S. D. Dunn and L. Konermann Biochemistry 42, 5896-5905 (2003). Online access. See also Biochemistry 42, 9248 (2003).

"Protein Folding Kinetics and Mechanisms Studied by Pulse-Labeling and Mass Spectrometry " L. Konermann and D. A. Simmons Mass Spectrom. Rev. 22, 1-26 (2003). Online access.

"Implementation of a Multifunctional Logic Gate Based on Folding/Unfolding Transitions of a Protein" A. S. Deonarine, S. M. Clark and L. Konermann Future Generation Computer Systems 19, 87-97 (2003). Online access.

"Diffusion Measurements by Electrospray Mass Spectrometry for Studying Solution-Phase Noncovalent Interactions" S. M. Clark and L. Konermann J. Am. Soc. Mass Spectrom. 14, 430-441 (2003). Online access.

Konermann, Lars (January 2003 ) "Protein Unfolding and Denaturants". In: Nature Encyclopedia of Life Sciences. London: Nature Publishing Group. Online access.

"Method and Apparatus for the Detection of Noncovalent Interactions by Mass Spectrometry-Based Diffusion Measurements" L. Konermann and S. M. Clark US Patent Application and PCT Application, filed May 30, 2003.

back to top



2002

"Characterization of Transient Protein Folding Intermediates During Myoglobin Reconstitution by Time-Resolved Electrospray Mass Spectrometry with On-Line Isotopic Pulse Labeling" D. A. Simmons and L. Konermann Biochemistry 41, 1906-1914 (2002). Online access.

"Taylor Dispersion Monitored by Electrospray Mass Spectrometry: A Novel Approach for Studying Diffusion in Solution" S. M. Clark, D. G. Leaist, and L. Konermann Rapid Commun. Mass Spectrom. 16, 1454-1462 (2002). Online access.

"Pre-Steady State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with On-Line Rapid-Mixing Techniques" L. Konermann and D. J. Douglas, Meth. Enzymol. 354 (Enzyme Kinetics and Mechanism, Part F), 50-64 (2002).

back to top



2001

"Electrochemically Induced pH Changes Resulting in Protein Unfolding in the Ion Source of an Electrospray Mass Spectrometer" L. Konermann, E. A. Silva, and O. F. Sogbein, Anal. Chem. 73, 4836-4844 (2001). Online access.

"From Small-Molecule Reactions to Protein Folding: Studying Biochemical Kinetics by Stopped-Flow Electrospray Mass Spectrometry" B. M. Kolakowski and L. Konermann Anal. Biochem. 292, 107-114 (2001). Online access.

back to top



2000

"Stopped-Flow-Electrospray Ionization Mass Spectrometry : A New Method for Studying Chemical Reaction Kinetics in Solution" B. M. Kolakowski, D. A. Simmons, and L. Konermann Rapid Commun. Mass Spectrom. 14, 772-776 (2000). Online access.

"The Effects of pH on the Kinetic Reaction Mechanism of Myoglobin Unfolding Studied by Time-Resolved Electrospray Ionization Mass Spectrometry" O. O. Sogbein, D. A. Simmons, and L. Konermann J. Am. Soc. Mass Spectrom. 11, 312-319 (2000). Online access.

"Method and apparatus for determining the rates of reactions in liquids by mass spectrometry " D. J. Douglas, L. Konermann, and B. A. Collings United States Patent  6054709, issued April 25, 2000.

back to top



1999

"Reconstitution of Acid-Denatured Holo-Myoglobin Studied by Time-Resolved Electrospray Ionization Mass Spectrometry", V. W. S. Lee, Y.-L. Chen, and L. Konermann  Anal. Chem. 71, 4154-4159 (1999). Online access.

"Monitoring Reaction Kinetics by Continuous-Flow Methods: The Effects of Convection and Molecular Diffusion Under Laminar Flow Conditions", L. Konermann, J. Phys. Chem. A 103, 7210-7216 (1999). Online access.

back to top



Earlier Work (1995-1998)

"Protein Folding Studied by Electrospray Ionization Mass Spectrometry", L. Konermann, Science Progress 81, 123-140 (1998).

"Unfolding of Proteins Monitored by Electrospray Ionization Mass Spectrometry: A Comparison of Positive and Negative Ion Modes", L. Konermann and D. J. Douglas, J. Am. Soc. Mass Spectrom. 9, 1248-1254 (1998).

"Equilibrium Unfolding of Proteins Monitored by Electrospray Ionization Mass Spectrometry: Distinguishing Two-State from Multi-State Transitions", L. Konermann and D. J. Douglas, Rapid Commun. Mass Spectrom. 12, 435-442 (1998).

"Pre-Steady State Kinetic Analysis of an Enzymatic Reaction Monitored by Time-Resolved Electrospray Ionization Mass Spectrometry", D. L. Zechel, L. Konermann, S. G. Withers, and D. J. Douglas, Biochemistry 37, 7664-7669 (1998).

"Stability of a Highly Charged Noncovalent Complex in the Gas Phase: Holomyoglobin", Y.-L. Chen, J. M. Campbell, B. A. Collings, L. Konermann, and D. J. Douglas, Rapid Commun. Mass Spectrom. 12, 1003-1010 (1998).

"Acid-Induced Unfolding of Cytochrome c at Different Methanol Concentrations: Electrospray Ionization Mass Spectrometry Specifically Monitors Changes in the Tertiary Structure", L. Konermann and D. J. Douglas, Biochemistry 36, 12296-12302 (1997).

"Acid-Induced Denaturation of Myoglobin Studied by Time-Resolved Electrospray Ionization Mass Spectrometry," L. Konermann, F. I. Rosell, A. G. Mauk, and D. J. Douglas, Biochemistry 36, 6448-6454 (1997).

"Cytochrome c Folding Kinetics Studied by Time-Resolved Electrospray Ionization Mass Spectrometry" L. Konermann, B. A. Collings, and D. J. Douglas  Biochemistry 36, 5554-5559 (1997).

"The Use of a Long-Lifetime Component of Tryptophan to Detect Slow Orientational Fluctuations of Proteins" K. Döring, W. Beck, L. Konermann, and F. Jähnig  Biophys. J. 72, 326-334 (1997).

"Pigment Assignment in the Absorption Spectrum of the Photosystem II Reaction Center by Site-Selection Fluorescence Spectroscopy" L. Konermann, I. Yruela,  and A. R. Holzwarth  Biochemistry 36, 7498-7502 (1997).

"Primary Processes and Structure of the Photosystem II Reaction Center. 5. Modeling of the Fluorescence Kinetics of the D1-D2-cyt-b559 Complex at 77 K" L. Konermann, G. Gatzen, and A. R. Holzwarth J. Phys. Chem. B 101, 2933-2944 (1997).

"Analysis of the Absorption Spectrum of Photosystem II Reaction Centers: Temperature Dependence, Pigment Assignment and Inhomogeneous Broadening" L. Konermann, and A. R. Holzwarth  Biochemistry 35, 829-842 (1996).

"A Long Lifetime Component in the Tryptophan Fluorescence of Some Proteins" K. Döring, L. Konermann,  T. Surrey,  and F. Jähnig,    Eur. Biophys. J. 23, 423-432 (1995).

back to top

Home | Lars Konermann | Research | People | Publications | News


Western University | Department of Chemistry | Department of Biochemistry