Question 3: How does developmental stage affect muscle substrate preference?


Recent work in our lab suggests that there is a difference in the reliance upon exogenous substrate to fuel muscle glycogen replenishment in juvenile fish (~5 g) compared to adult fish (> 100 g) after a bout of exhaustive exercise (S. Wu, 450a/452b project). If juvenile fish are not fed after a bout of exercise, then their muscle glycogen stores are not replenished to pre-exercise levels. In fact, they are lower 24h post-exercise than at rest. Furthermore, if fed, muscle glycogen levels are replenished within 4-6 hours. These observations clearly suggest that muscle from juvenile fish is dependent upon exogenous substrate (e.g. substate from the blood) for glycogen resynthesis, but that does not appear to be the case in adult fish.

There is lots to do in this area as these observations are just the begining. The whole field of developmental physiology of fish in relation to muscle fuel use is wide-open


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