Name: John
Alan Kiernan. Born: 1942; Kidderminster, Worcestershire,
England. Married: 1967 to Tessa Mary Westcott; 5
children; 11 grandchildren. Job(s): Retired from teaching
neuroanatomy
to medical and other students. Research in neuroscience and in
developing and
improving histological and histochemical techniques. Occasional
consultant for medical devices companies, patent law firms, publishers
and universities, mostly in USA, also Asia.
Secretary and a
trustee of the Biological Stain Commission. Resides in:
St Thomas, Ontario, Canada. (This is half-way between London and the North
Shore of Lake Erie, and roughly equidistant from
Toronto, Buffalo and Detroit, all about a 150 minutes drive away.) Other interests: Field botany (vascular
plants).
Education:
1953-1960:
King
Edward VI Grammar School, Five Ways, Birmingham, England.
1960-1966
and 1967-1969:
University
of Birmingham, England.
Degrees etc.
1963:
BSc
(Anatomical Studies) University of Birmingham, England.
1966:
MB, ChB
University of Birmingham, England.
1966:
MRCS
England; LRCP London.
1969:
PhD
(Faculty of Medicine) University of Birmingham, England. Thesis: Studies on Nervous Regeneration.
1969:
MA
(conferred by statute as a condition of appointment) University of
Cambridge, England.
1979:
DSc (by
thesis, Studies in Neurohistology and Related Fields, through Department of Anatomy) University of Birmingham,
England.
Employment:
1966: House Surgeon, Accident Unit, East Birmingham
Hospital,
Birmingham, England.
1967: House Physician, Worcester Royal Infirmary, Worcester,
England.
1967-1969:
Research Fellow (MRC), Department of Anatomy, University of
Birmingham, England.
1967-1972: General medical practice, part
time, Birmingham, Cambridge etc.
1969-1971: Stanley Elmore Senior Research Fellow,
Sidney Sussex
College, Cambridge, England.
1970-1972: Director of Studies in Medicine, Sidney
Sussex College,
Cambridge, England.
1971-1972: University Demonstrator, Department of
Anatomy, University
of Cambridge; College Lecturer and Fellow, Sidney Sussex College,
Cambridge, England.
1972-1975: Assistant Professor, Department of
Anatomy, The University
of Western Ontario, London, Canada.
1975-1981: Associate Professor, Department of
Anatomy, The University
of Western Ontario (Tenure, 1975).
1981-2011:
Professor, Department of Anatomy, The University of Western
Ontario, London, Canada.
1985-1986:
Commonwealth Medical Fellow, and
visiting professor, Department of Anatomy
and Cell
Biology, The University of Sheffield,
England.
1990-2011:
Core faculty member, Neuroscience Graduate Programme, The
University of Western Ontario, London, Canada.
2011-
:
Professor
Emeritus, Department of Anatomy & Cell Biology, The University
of
Western Ontario, London, Canada.
Membership of societies, editorial boards etc.
1957- : Botanical
Society of the British Isles (Member)
1958- 2023: Birmingham
Natural History Society (Member)
1967-2016: Principal
List (Licence to Practise), General Medical Council (U.K.)
1968- : Anatomical
Society of Great Britain and Ireland (Member; Senior Member 2008-
)
1969-2009: Cambridge
Philosophical Society (Fellow)
1974-1981: Canadian Association of
Anatomists (Member)
1976- : The
Histochemical Society (Member)
1980-1987: Society for Neuroscience
(Member)
1982- : Southern
Ontario Neuroscience Association (Member)
1985-2009: British
Neuroscience Association (formerly Brain Research Association) (Member)
1985-2009: Royal
Microscopical Society (Fellow)
1987-2011: Canadian
Association for Neuroscience (Member)
1993- : Biological
Stain Commission (Member; also a Trustee since 1995; Secretary and
webmaster, 2008- )
1995- : Field
Botanists of Ontario (Member)
1998-
: Society for
Histotechnology (Member)
1995- :
Editorial
Board of Biotechnic and
Histochemistry
1998-2000
and 2006- : Editorial Board of The
Journal of Histotechnology
2006- : Associate Editor, The
Journal of Histotechnology
Undergraduate teaching
(University of Birmingham)
Medical
students, 3rd year.
Neuroanatomy Demonstrator in an integrated Neurology course;
3 hr per week. 1963-1965 and 1967-1969.
Undergraduate teaching
(University of Cambridge)
Medical
students, 2nd year.
Lectures, laboratory and tutorial teaching in Anatomy,
Histology, Neuroanatomy and Clinical Pharmacology.
6 hr per
week. 1969-1972.
Undergraduate teaching
(University of Western Ontario):
Medical students, 1st/2nd year.
Neuroanatomy (as part of an integrated Neurosciences course); 45 hours
lectures and laboratory (1972-1997).
Physiotherapy, Occupational Therapy and some others.
Anatomy 250 (Neuroanatomy). 52 hours lectures & laboratory
(1978-1997); also Anatomy 350a (Neuroanatomy): occasional
contributions, mainly laboratory, about 15 hours
(1997-2002); course manager and principal instructor, 42 hrs
(2002-2006 ).
Graduate courses taught (University of Western Ontario):
Anatomy 530a. Human
Neuroanatomy. Occasional contributions, mainly laboratory;
about 15 hours (1994-2001) ; course manager and principal instructor,
42 hrs (2002-2006 ).
Anatomy 531a/b. Advanced Human Neuroscience (until 1997).
About 20 hours, tutorial teaching.
Anatomy 540. Research Methods in Anatomy (1997-2002).
Coordinator, and 10 hours lectures, 4-12 hours laboratory.
Anatomy 543a/b. Histochemistry (1976-2004). Coordinator and
sole teacher, about 20 hours tutorial teaching and 10-15 hours
laboratory.
Anatomy 545y. Human Neuroanatomy (until 1994). Coordinator,
and 16 hours lectures.
Anatomy 535b. Anatomical Foundations of Neuroscience (1998-
2011). Coordinator and sole teacher. 36 hours lectures and
laboratory Number changed to 9535b in 2008..
Neuroscience 500. Principles of Neuroscience. (1996-2008).
Systems section: Structural basis of nervous system circuitry; One
lecture, 2 hours.
Black
for Axonal
regeneration, Neuropathology and Blood-tissue
barriers. (This includes papers concerned
with interactions between
vascular permeability and axonal regeneration.)
Blue for
method-related
Histological
and histochemical stuff.
(This
category includes
papers in
which my interest is in a technique, even though this may not be what
the paper is mainly about.)
Brown
for
Trophic interactions of neurons and other cells, especially muscle.
Green
for
Botany.
Magenta
for
Mast
cells. (Most of
these concern
interactions of mast cells with neurons.)
Red
for
normal Neuroanatomy
and Neurohistology (Most of these items are
textbooks or other
teaching publications.)
This
Orange color
is
for Other
things that do
not fit in any of the above categories.
There are not many of these.
A note on multiple
authorship of
papers in peer-reviewed journals. Most
of the publications listed in my CV have 1, 2 or3 authors, all equal
contributors by virtue of supervision, doing the lab work and producing
the manuscript. Usually the first author did most of the
lab work and the last author was the supervisor who wrote the paper and
responded to the referees' reports. For some papers in this
list,
especially before about 1990, the authors are listed
alphabetically. This was an admirable principle held by the Journal of Physiology
for several decades, which I tried to encourage among collaborators in
papers submitted to all journals. (I'm an author of only one paper in
J. Physiol.;
I did some of the
lab work. Happily the supervisor of the
project had a surname beginning with T and retained his last-author
position.) I support the notion that authors should be equal
contributors, whereas minor
contributions (academic, financial, technical etc) should be
recognized
in acknowledgements.
There is no generally agreed formula that determines the order in a
list of "authors" of a scientific paper. I appear as a
"middle"
author in some of the following items, including at least three for
which I deserve only an acknowledgement. For a few,
I'm in
the middle of a list of three, having supervised two students
with surnames before and after K in the alphabet.
Papers
in peer-reviewed journals
Holmes,
R. L.
and Kiernan, J. A. 1964. The fine structure of the infundibular process
of the hedgehog. Zeitschrift fur Zellforschung 61, 894-912. https://doi.org/10.1007/BF00340042
Kiernan,
J. A. 1964. Carboxylic esterases of the hypothalamus and
neurohypophysis of the hedgehog. Journal of the Royal Microscopical
Society 83, 297-306.
Campbell,
D. J. and Kiernan, J. A. 1966. Mast cells in the central nervous
system. Nature 210, 756-757. https://doi.org/10.1038/210756b0
Kiernan,
J. A. 1966. Notes on the flora of Worcestershire: additions to the
flora since 1909. Proceedings of the Birmingham Natural History Society
20, 19-37.
Kiernan,
J. A.
1967. On the probable absence of retino-hypothalamic connections in
five mammals and an amphibian. Journal of Comparative Neurology 131,
405-408.
Kiernan,
J. A.
1968. Effect of interrupting retino-hypothalamic connections on the
melanophore response in Xenopus laevis. Experientia 24, 503-504. https://doi.org/10.1007/BF02144419
Kiernan,
J. A. 1968. Notes on the flora of Worcestershire. II. Further additions
to the flora since 1909. Proceedings of the Birmingham Natural History
Society 21, 83-88.
Kiernan,
J. A. 1970. Silver staining of axons in subcellular fractions of
nervous tissue. Experientia 26, 1352. https://doi.org/10.1007/BF02113026
Kiernan, J.
A. 1971. Pituicytes and the regenerative properties of neurosecretory
and other axons in the rat. Journal of Anatomy 109, 97-114.
Kiernan, J.
A. and Rawcliffe, P. M. 1971. Effects of triiodothyronine on the
cerebellar cortex of the new-born rat in tissue culture. Experientia
27, 678-679. https://doi.org/10.1007/BF02136957
Kiernan, J.
A. and Pettit, D. R. 1971. Organ culture of the central nervous system
of the adult rat. Experimental Neurology 32, 111-120. https://doi.org/10.1016/0014-4886(71)90169-5
Fertig, A.,
Kiernan, J. A. and Seyan, S. S. A. S. 1971. Enhancement of axonal
regeneration in the brain of the rat by corticotrophin and
triiodothyronine. Experimental Neurology 33, 372-385. https://doi.org/10.1016/0014-4886(71)90029-X
Kiernan,
J. A. 1971. Rhynchosinapis - The Worcestershire records. Watsonia -
Journal of the Botanical Society of the British Isles 8, 293.
Kiernan, J.
A. 1972. The involvement of mast cells in vasodilatation due to axon
reflexes in injured skin. Quarterly Journal of Experimental Physiology
57, 311-317.
Kiernan, J.
A. 1972. Effects of known and suspected neurotransmitter substances and
of some nucleotides on isolated mast cells. Experientia 28, 653-655. https://doi.org/10.1007/BF01944958
Stoddart,
R. W. and Kiernan, J. A. 1972. Histochemical demonstration of the
α-D-arabinopyranoside configuration with fluorescent-labelled
concanavalin A. Histochemie 33 87-94. https://doi.org/10.1007/BF00304230
Kiernan,
J. A. and Stoddart, R. W. 1973. Fluorescent-labelled aprotinin: a new
reagent for the histochemical detection of acid mucosubstances.
Histochemie 34, 77-84. https://doi.org/10.1007/BF00304309
Stoddart,
R. W. and Kiernan, J. A. 1973. Aprotinin: a carbohydrate-binding
protein. Histochemie 34, 275-280. https://doi.org/10.1007/BF00306299
Drayton, M.
R. and Kiernan, J. A. 1973. Effects of aprotinin on organ cultures of
the cerebellum of the adult rat. Experimental Neurology 39, 381-388. https://doi.org/10.1016/0014-4886(73)90024-1
Cockett, S.
A. and Kiernan, J. A. 1973. Acceleration of peripheral nervous
regeneration in the rat by exogenous triiodothyronine. Experimental
Neurology 39, 389-394. https://doi.org/10.1016/0014-4886(73)90025-3
Heap, B. J.
and Kiernan, J. A. 1973. Histological, histochemical and
pharmacological observations on mast cells in the stomach of the rat.
Journal of Anatomy 115, 315-325. PMCID: PMC1271487
Pettit, D. R.
and Kiernan, J. A. 1974. Experimental allergic encephalomyelitis in the
rat: histopathological studies and the effects of sera on adult
cerebellar organ cultures. Acta Neuropathologica 27, 83-91. https://doi.org/10.1007/BF00687243
Kiernan,
J. A. 1974. Effects of metabolic inhibitors on vital staining with
methylene blue. Histochemistry 40, 51-57. https://doi.org/10.1007/BF00490273
Kiernan,
J. A.
and Mitchell, R. 1974. Some observations on the innervation of the
pinna of the ear of the rat. Journal of Anatomy 117, 397-402. PMCID: PMC1231412
Kiernan, J.
A. 1974. Effects of insulin, triiodothyronine and corticosterone on
organ cultures of the adult rat cerebellum. Acta Neuropathologica 30,
25-31. https://doi.org/10.1007/BF00685319
Kiernan,
J. A. and McIsaac, G. 1974. Complete staining of neuromuscular
innervation with bromoindigo and silver. Stain Technology 49, 211-214. https://doi.org/10.3109/10520297409116980
Kiernan, J.
A. 1974. The development of mast cells in vitro. Journal of Anatomy
118, 517-529. PMCID: PMC1231550
Kiernan, J.
A. 1974. Action of adenosine triphosphate on mast cells in normal and
denervated skin. Archiv fur Dermatologische Forschung 251, 83-86. https://doi.org/10.1007/BF00561714
Kiernan,
J. A. 1975. Localization of α-D-glucosyl and α-D-mannosyl groups of
mucosubstances with concanavalin A and horseradish peroxidase.
Histochemistry 44, 39-45. https://doi.org/10.1007/BF00490419
Kiernan, J.
A. 1975. A pharmacological and histological investigation of the
involvement of mast cells in cutaneous axon reflex vasodilatation.
Quarterly Journal of Experimental Physiology 60, 123-130. https://doi.org/10.1113/expphysiol.1975.sp002298
McIsaac, G.
and Kiernan, J. A. 1975. Accelerated recovery from peripheral nerve
injury in experimental hyperthyroidism. Experimental Neurology 48,
88-94. https://doi.org/10.1016/0014-4886(75)90224-1
McIsaac, G.
and Kiernan, J. A. 1975. Acceleration of neuromuscular reinnervation by
triiodothyronine. Journal of Anatomy 120, 551-560. PMCID: PMC1231695
Heap, B. J.
and Kiernan, J. A. 1975. Mast cells and gastric secretion in the rat.
Quarterly Journal of Experimental Physiology 60, 307-313.
Davis, H.,
Gascho, C. and Kiernan, J. A. 1975. Action of aprotinin on the survival
of adult cerebellar neurons in organ culture. Acta Neuropathologica 32,
359-362. https://doi.org/10.1007/BF00696799
Davis, H.,
Gascho, C, and Kiernan, J. A. 1975. The skin in magnesium-deficient
rats. Archives of Dermatological Research 254, 1-13. https://doi.org/10.1007/BF00561528
Davis, H.,
Gascho, C. and Kiernan, J. A. 1976. Action of aprotinin on organ
cultures of the rat's kidney. In Vitro 12, 192-197. https://doi.org/10.1007/BF02796441
Kiernan, J.
A. 1976. A comparative survey of the mast cells of the mammalian brain.
Journal of Anatomy 121, 303-311. PMCID: PMC1231801
Kiernan, J.
A. 1976. Evidence for the involvement of vasoactive constituents of
mast cells in axon reflex vasodilatation in the skin of the rat.
Archives of Dermatological Research 255, 1-8. https://doi.org/10.1007/BF00581672
Cook, R. A.
and Kiernan, J. A. 1976. Effects of triiodothyronine on protein
synthesis in regenerating peripheral neurons. Experimental Neurology
52, 515-524. https://doi.org/10.1016/0014-4886(76)90222-3
Lampe, M.
and Kiernan, J. A. 1976. Degranulation of dermal mast cells: effects of
fixation and of antidromic nervous impulses on two histochemically
identified cell-types. Archives of Dermatological Research 257, 125-129. https://doi.org/10.1007/BF00558085
Lampe, M.
and Kiernan, J. A. 1977. Cytochemical variation of mast cells in the
skin of the rat. Archives of Dermatological Research 258, 69-80. https://doi.org/10.1007/BF00582869
Kiernan, J.
A. 1977. A study of chemically induced inflammation in the skin of the
rat. Quarterly Journal of Experimental Physiology 62, 151-161.
Kiernan,
J. A. 1977. Histochemical demonstration of unsaturated hydrophilic
lipids with palladium chloride. Journal of Histochemistry and
Cytochemistry 25, 200-205.
Stelmack, B.
M. and Kiernan, J. A. 1977. Effects of triiodothyronine on the normal
and regenerating facial nerve of the rat. Acta Neuropathologica 40,
151-155. https://doi.org/10.1007/BF00688704
Heinicke, E.
A. and Kiernan, J. A. 1978. Vascular permeability and axonal
regeneration in skin autotransplanted into the brain. Journal of
Anatomy 125, 409-420. PMCID: PMC342472
Mervart, M.
and Kiernan, J. A. 1978. Axonal regeneration in experimental allergic
peripheral neuritis. Acta Neuropathologica 41, 197-200. https://doi.org/10.1007/BF00690435
Kiernan, J.
A. 1978. An explanation of axonal regeneration in peripheral nerves and
its failure in the central nervous system. Medical Hypotheses 4, 15-26. https://doi.org/10.1016/0306-9877(78)90022-1
Kiernan,
J. A. 1978. Recovery of osmium tetroxide from used fixative solutions.
Journal of Microscopy 113, 77-82.
Kiernan, J.
A. 1979. Production and life span of cutaneous mast cells in young
rats. Journal of Anatomy 128, 225-238. PMCID: PMC1232929
Sparrow, J.
R. and Kiernan, J. A. 1979. Uptake and retrograde transport of proteins
by regenerating axons. Acta Neuropathologica 47, 39-47. https://doi.org/10.1007/BF00698271
Kiernan, J.
A. 1979. Hypotheses concerned with axonal regeneration in the mammalian
nervous system. Biological Reviews 54, 155-197.
Davis, H.
L. and Kiernan, J. A. 1980. Neurotrophic effects of sciatic nerve
extract on denervated extensor digitorum longus muscle in the rat.
Experimental Neurology 69, 124-134. https://doi.org/10.1016/0014-4886(80)90148-X
Kiernan, J.
A. and Contestabile, A. 1980. Vascular permeability associated with
axonal regeneration in the optic system of the goldfish. Acta
Neuropathologica 51, 39-45. https://doi.org/10.1007/BF00688848
Sparrow, J.
R. and Kiernan, J. A. 1981. Endoneurial vascular permeability in
degenerating and regenerating peripheral nerves. Acta Neuropathologica
53, 181-188. https://doi.org/10.1007/BF00688020
Davis, H. L.
and Kiernan, J. A. 1981. Effect of nerve extract on atrophy of
denervated or immobilized muscles. Experimental Neurology 72, 582-591. https://doi.org/10.1016/0014-4886(81)90007-8
Doucette, J.
R., Kiernan, J. A. and Flumerfelt, B. A. 1983. Two different patterns
of retrograde degeneration in the olfactory epithelium following
transection of primary olfactory axons. Journal of Anatomy 136, 673-689. PMCID: PMC6886621
Doucette, J.
R., Kiernan, J. A. and Flumerfelt, B. A. 1983. The reinnervation of
olfactory glomeruli following transection of primary olfactory axons in
the central or peripheral nervous system. Journal of Anatomy 137, 1-19. PMCID: PMC6630027
Davis, H.
L. and Kiernan, J. A. 1984. Effect of nerve extract on number of
acetylcholine receptors in denervated muscles of rats. Experimental
Neurology 83, 108-117. https://doi.org/10.1016/0014-4886(84)90050-5
Kiernan,
J. A. 1984. Chromoxane cyanine R. I. Physical and chemical properties
of the dye and of some of its iron complexes. Journal of Microscopy
134, 13-23.
Kiernan, J.
A. 1985. A conditioning lesion does not promote axonal regeneration in
the optic nerve of the rat. Experimental Neurology 87, 181-184. https://doi.org/10.1016/0014-4886(85)90145-1
Davis, H.
L., Heinicke, E. A., Kiernan, J. A. and Cook, R. A. 1985. Partial
purification from mammalian peripheral nerve of a trophic factor which
ameliorates atrophy of denervated muscle. Experimental Neurology 89,
159-171. https://doi.org/10.1016/0014-4886(85)90273-0
Kiernan,
J. A. 1986. The action of chromium(III) in fixation of animal tissues.
Histochemical Journal 17, 1131-1146. https://doi.org/10.1007/BF01002538
Kiernan,
J. A.
1986. "Pre"-embryos. (Letter, opposing experimentation with human
embryos) Nature 321, 376. https://doi.org/10.1038/321376b0
Kiernan, J.
A. 1986. Axonal and vascular changes following injury to the rat's
optic nerve. Journal of Anatomy 141, 139-154. PMCID: PMC1166396
Flumerfelt,
B. A., Kiernan, J. A., Krcek, J. P. and Sholdice, J. 1986.
Reinnervation of skeletal muscle in the tongue by preganglionic
parasympathetic vagal neurons. Journal of Anatomy 146, 117-130. PMCID: PMC1166528
Heinicke,
E. A., Kiernan, J. A. and Wijsman, J. 1987. Specific, selective and
complete staining of neurons of the myenteric plexus, using cuprolinic
blue. Journal of Neuroscience Methods 21, 45-54. https://doi.org/10.1016/0165-0270(87)90101-4
Kiernan, J.
A. 1988. Intestinal anastomosis in the rat facilitated by a rapidly
digestible internal splint and indigestible but absorbable sutures.
Journal of Surgical Research 45, 427-431. https://doi.org/10.1016/0022-4804(88)90192-8
Hudson, A. J.
and Kiernan, J. A. 1988. Preservation of certain voluntary muscles in
motor neurone disease. (Letter, concerning transneuronal degeneration
in MND) Lancet 1988-I, 331: 652-653. https://doi.org/10.1016/S0140-6736(88)91455-9
Barr, D.
B., Duncan, J. A., Kiernan, J. A., Soper, B. D. and Tepperman, B. L.
1988. Binding and biological actions of prostaglandin E2 and I2 in
cells isolated from rabbit gastric mucosa. Journal of Physiology 405,
39-55. https://doi.org/10.1113/jphysiol.1988.sp017320
Kiernan,
J. A. 1989. North American Solidago species in Central England.
Proceedings of the Birmingham Natural History Society 26, 22-24.
Kiernan, J.
A. and Heinicke, E. A. 1989. Sennosides do not kill myenteric neurons
in the colon of the rat or mouse. Neuroscience 30, 837-842. https://doi.org/10.1016/0306-4522(89)90175-9
Tepperman,
B. L., Kiernan, J. A. and Soper, B. D. 1989. The effect of
sialoadenectomy on gastric mucosal integrity in the rat: roles of
epidermal growth factor and prostaglandin E2. Canadian Journal of
Physiology and Pharmacology 67, 1512-1519. https://doi.org/10.1139/y89-244https://doi.org/10.1139/y89-244
Heinicke, E.
A. and Kiernan, J. A. 1990. Resistance of myenteric neurons in the
rat's colon to depletion by 1,8-dihydroxyanthraquinone. Journal of
Pharmacy and Pharmacology 42, 123-125. https://doi.org/10.1111/j.2042-7158.1990.tb05366.x
Heinicke,
E. A.
and Kiernan, J. A. 1990. An immunohistochemical study of the myenteric
plexus of the colon in the rat and mouse. Journal of Anatomy 170, 51-62. PMCID: PMC1257062
Kiernan, J.
A. 1990. Degranulation of mast cells in the trachea and bronchi of the
rat following stimulation of the vagus nerve. International Archives of
Allergy and Applied Immunology 91, 398-402. https://doi.org/10.1159/000235149
Kiernan,
J. A.
1990. Frozen "pre-embryos". (Letter, deprecating the use of misleading
terminology) JAMA - Journal of the American Medical
Association
264,
2283. https://doi.org/10.1001/jama.1990.03450180038014
Kiernan,
J. A.
1990. Interactions between mast cells and nerves. Neurogenic
inflammation. (Letter, commenting on a recent TIPS review article)
Trends in Pharmacological Sciences 11, 316. PDF download link
Kiernan, J.
A. and Hudson, A. J. 1991. Changes in sizes of cortical and lower motor
neurons in amyotrophic lateral sclerosis. Brain 114, 843-853. https://doi.org/10.1093/brain/114.2.843
Tepperman,
B. L., Besco, J. M., Kiernan, J. A. and Soper, B. D. 1991. Relationship
between myeloperoxidase activity and the ontogenic response of rat
gastric mucosa to ethanol. Canadian Journal of Physiology and
Pharmacology 69, 1882-1888. https://doi.org/10.1139/y91-278
Pringle, C.
E., Hudson, A. J., Munoz, D. G., Kiernan, J. A., Brown, W. F. and
Ebers, G. C. 1992. Primary lateral sclerosis. Clinical features,
neuropathology and diagnostic criteria. Brain 115, 495-420. https://doi.org/10.1093/brain/115.2.495
Hudson, A.
J., Kiernan, J. A., Munoz, D. G., Pringle, C. E., Brown,W. F. and
Ebers, G. C. 1993. Clinicopathological features of primary lateral
sclerosis are different from amyotrophic lateral sclerosis. Brain
Research Bulletin 30, 359-364. https://doi.org/10.1016/0361-9230(93)90265-D
Kiernan, J.
A. and Hudson, A. J. 1993. Changes in shapes of surviving motor neurons
in amyotrophic lateral sclerosis. Brain 116, 203-215. https://doi.org/10.1093/brain/116.1.203
Kiernan, J.
A. and Hudson, A. J. 1993. Anti neurone antibodies are not
characteristic of amyotrophic lateral sclerosis. NeuroReport 4, 427-430. https://doi.org/10.1097/00001756-199304000-00022
Allen, D. T.
and Kiernan, J. A. 1994. Permeation of proteins from the blood and
peripheral nerves into ganglia. Neuroscience 99, 755-764. https://doi.org/10.1016/0306-4522(94)90192-9
Savedia, S.
and Kiernan, J. A. 1994. Increased synthesis of ubiquitin mRNA in motor
neurons after axotomy. Neuropathology and Applied Neurobiology 20,
577-586.
Kiernan,
J. A. 1996. Review of current silver impregnation: Techniques for
histological examination of skeletal muscle innervation. Journal of
Histotechnology 19, 257-267.
Kiernan,
J. A. 1996. Staining paraffin sections without prior removal of the
wax. Biotechnic and Histochemistry 71, 304-310. https://doi.org/10.3109/10520299609117180
Kiernan,
J. A., Macpherson, C. M., Price, A. and Sun, T. 1998. A histochemical
examination of the staining of kainate-induced neuronal degeneration by
anionic dyes. Biotechnic and Histochemistry 73, 244-254. https://doi.org/10.3109/10520299809141118
Conway,
K. and Kiernan, J. A. 1999. Chemical dehydration of specimens with
2,2-dimethoxypropane (DMP) for paraffin processing of animal tissues:
practical and economic advantages over dehydration in ethanol.
Biotechnic and Histochemistry 74, 20-26. https://doi.org/10.3109/10520299909066473
Canham,
P. B., Finlay, H. M., Kiernan, J. A. and Ferguson, G. G. 1999. Layered
structure of saccular aneurysms assessed by collagen birefringence.
Neurological Research 21, 618-626. https://doi.org/10.1080/01616412.1999.11740986
von
Bohlen und Halbach, O. and Kiernan, J. A. 1999. Diaminobenzidine
induces fluorescence in nervous tissue, providing intrinsic
counterstaining of sections prepared for peroxidase histochemistry.
Biotechnic and Histochemistry 74, 236-243. https://doi.org/10.3109/10520299909034659
Wu, M.
& Kiernan, J. A. 2001. A new method for surface staining large
slices of fixed brain, using a copper phthalocyanine dye. Biotechnic
& Histochemistry 76, 253-255. https://doi.org/10.1080/10520290802475878
Kiernan,
J. A. 2002. Silver staining for spirochetes in tissues: rationale,
difficulties, and troubleshooting. Laboratory Medicine 33, 705-708. Click here for link to PDF file
Conway,
K., Pin, C. & Kiernan, J. A. & Merrifield, P. 2004. The
E-protein HEB is preferentially expressed in developing muscle.
Differentiation 72, 327-340. https://doi.org/10.1111/j.1432-0436.2004.07207004.x
Bova,
G.
S., Eltoum, I. A., Kiernan, J. A., Siegal, G. P., Frost, A. R., Best,
C. J. M., Gillespie, J. W., Su, G. H. & Emmert-Buck, M. R.
2005. Optimal molecular profiling of tissue and tissue components:
defining the best processing and microdissection methods for biomedical
applications. Molecular Biotechnology 29, 119-152. https:/10.1385/MB:29:2:119
Kiernan,
J. A. 2006. Dyes and
other colorants in microtechnique and biomedical research. Coloration
Technology 122, 1-21. Click here for PDF file
Dapson,
R. W., Frank, M. & Kiernan, J. A. 2006. Revised procedures for
the
certification of carmine (CI 75470, Natural red 4) as a biological
stain. Biotechnic & Histochemistry 82:
13-15. https://doi.org/10.1080/10520290701207364
Frank,
M., Dapson, R. W.,
Wickersham, T. W. & Kiernan, J. A. 2007. Certification
procedures for nuclear fast red (Kernechtrot), C.I. 60760.
Biotechnic &
Histochemistry82:
35-39. https://doi.org/10.1080/10520290701333475
Kiernan,
J. A. 2007. Histochemistry of staining methods for normal and
degenerating myelin in the central and peripheral nervous systems.
Journal of Histotechnology 30: 87-106. https://doi.org/10.1179/his.2007.30.2.87
Kiernan,
J. A. 2007.
Indigogenic substrates for the detection and localization of
enzymes. Biotechnic
&
Histochemistry82:
73-103. https://doi.org/10.1080/10520290701375278
Kiernan,
J. A. 2009. A system for quantitative evaluation of morphological
effects of fixatives for light microscopy, using paraffin sections of
kidney and brain. Biotechnic & Histochemistry 84: 1-10. https://doi.org/10.1080/10520290802646619
Kiernan,
J. A. 2009. Graduate training and education in histotechnology and
histochemistry. Journal of
Histotechnology 32: 115-118. https://doi.org/10.1179/his.2009.32.3.115
Dapson,
R., Horobin, R. W. & Kiernan, J. A. 2010. Hematoxylin
shortages:
their causes and duration, and other dyes that can replace hemalum in
routine H & E staining. Biotechnic & Histochemistry
85: 55-63. https://doi.org/10.3109/10520290903048400
Dapson,
R. W., Fagan, C., Kiernan, J. A. & Wickersham, T. W. 2011.
Certification procedures for sirius red F3B (CI 35780, Direct red 80).
Biotechnic & Histochemistry 86: 133-139. https://doi.org/10.3109/10520295.2011.570277
Kiernan,
J. A. 2012. Anatomy of the temporal lobe. Epilepsy Research and
Treatment Vol. 2012: Article ID 176157, 12 pages. https://doi.org/10.1155/2012/176157.
Owen,
M. A., Schauwers, W., Hugh-Jones, M. E., Kiernan, J. A., Turnbull, P.
C. B. & Beyer, W. 2013. A simple, reliable M’Fadyean
stain
for visualizing the Bacillus
anthracis capsule. Journal of Microbiology
Methods 92: 264-269. https://doi.org/10.1016/j.mimet.2013.01.009
Kiernan,
J. A. 2018. Does progressive nuclear staining with hemalum (alum
hematoxylin) involve DNA, and what is the nature of the dye-chromatin
complex? Biotechnic & Histochemistry 92 (2): 133-148.
https://doi.org/10.1080/10520295.2017.1399466
Dunmore-Buyze,
P. J., Cruje, C., Nong, Z., Lee, J., Kiernan, J. A., Pickering, J. G.
& Drangova, M. 2019. 3D vessel-wall virtual histology of whole-body
perfused mice using a novel heavy element stain. Scientific
Reports 9(1): Article number 698. https://doi.org/10.1038/s41598-018-36905-z
Fagan,
C., Dapson, R. W., Horobin, R. W. & Kiernan, J. A. 2020. Revised
tests and standards for certification of alcian blue dyes by the
Biological Stain Commission. Biotechnic &
Histochemistry95(5): 333-340. https://doi.org/10.1080/10520295.2019.1699163
Ali,
S., Dapson, R. W., Kiernan, J. A., Horobin, R. W.
& Kazlaunicias, A. 2022. At least four distinct blue cationic
phthalocyanine dyes are sold as “alcian blue”. So, what is alcian
blue? Biotechnic & Histochemistry 97(1): 11-22. https://doi.org/10.1080/10520295.2021.2018497
Kiernan,
J. A. 1981. Histological and Histochemical Methods: Theory and
Practice. Oxford: Pergamon Press.
Barr,
M. L. and
Kiernan, J. A. 1983. The Human Nervous System. An Anatomical Viewpoint.
4th edition. Philadelphia: Harper and Row.
Kiernan,
J. A.
1987. An Introduction to Human Neuroscience. Philadelphia: Lippincott.
Barr,
M. L. and
Kiernan, J. A. 1988. The Human Nervous System. An Anatomical Viewpoint.
5th edition. Philadelphia: Lippincott.
Kiernan,
J. A. 1990. Histological and Histochemical Methods: Theory and
Practice. 2nd edition. Oxford: Pergamon Press.
Barr,
M. L. and
Kiernan, J. A. 1993. The Human Nervous System. An Anatomical Viewpoint.
6th edition. Philadelphia: Lippincott.
Kiernan,
J. A.
1998. Barr's The Human Nervous System: An Anatomical Viewpoint. 7th
edition. Philadelphia: Lippincott-Raven.
Kiernan,
J. A. 1999. Histological and Histochemical Methods: Theory and
Practice. 3rd edition. Oxford: Butterworth-Heinemann. (Paperback
edition published in 2000 by Arnold Publishers, London)
Horobin,
R. W. and Kiernan, J. A. (Eds) 2002. Conn's Biological Stains. A
Handbook of Dyes and Fluorochromes for Use in Biology and Medicine.
10th ed. Oxford: BIOS Scientific Publishers (ISBN 1859960995).
Kiernan,
J. A. and Mason, I. G. (Eds) 2002. Microscopy and Histology for
Molecular Biologists. A User's Guide. London: Portland Press (ISBN
1855781417 hard cover, 1855781565 paperback).
Kiernan,
J. A.
2005. Barr's The Human Nervous System: An Anatomical Viewpoint. 8th
edition. Baltimore: Lippincott, Williams & Wilkins.
Kiernan,
J. A. 2008. Histological and Histochemical Methods: Theory and
Practice. 4th edition. Bloxham, UK: Scion Publishing. (ISBN
9781904842422)
Kiernan, J. A.
2009. Barr's The Human Nervous System: An Anatomical Viewpoint. 9th
edition. Baltimore: Lippincott, Williams & Wilkins (ISBN
9780781782562).
Kumar,
G. L. and Kiernan, J. A. (Eds) 2010. Education Guide: Special Stains
and H
& E. 2nd ed. Carpinteria, CA: Dako North America. 300 pages.
Download from
https://www.agilent.com/cs/library/technicaloverviews/public/08066_special_stains_eduguide.pdf
Kiernan,
J. A. & Rajakumar, N. 2014. Barr's The Human Nervous
System:
An Anatomical Viewpoint. 10th edition. Baltimore: Lippincott, Williams
& Wilkins (ISBN 9781451173277).
Kiernan,
J. A. 2015. Histological and Histochemical Methods: Theory and
Practice. 5th edition. Banbury, UK: Scion Publishing. (ISBN
9781907904325).
Kiernan,
J. A. & Berry, M. 1975. Neuroanatomical methods. Chapter 1 in
Methods
in Brain Research, ed. P. B. Bradley. London: John Wiley. pp. 1-77.
Kiernan, J.
A. 1984. Role of mast cells in vascular responses due to axon reflexes
in injured skin. In Antidromic Vasodilatation and Neurogenic
Inflammation, ed. L. A. Chahl, J. Szolczanyi and F. Lembeck. Budapest:
Akademiai Kiado. pp. 223-243.
Kiernan,
J. A.
1992. Some functional pathways in the central nervous system. Chapter
450 in Textbook of Internal Medicine, 2nd ed., ed. W. N. Kelley, Vol.
2, pp. 2138-2145. Philadelphia: Lippincott.
Kiernan,
J. A.
1997. Some functional pathways in the central nervous system. Chapter
17 in Textbook of Internal Medicine, 3rd ed., ed. W. N. Kelley, pp.
94-102. Philadelphia: Lippincott-Raven.
Kiernan,
J. A.
2000. Some functional pathways in the central nervous system. Chapter
14 in Kelley's Textbook of Internal Medicine, 4th ed., ed. H. D. Humes,
pp. 99-108. Philadelphia: Lippincott, Williams and Wilkins.
Kiernan,
J. A. 2002. Freezing and fixation. Chapter 8 in Microscopy and
Histology for Molecular Biologists. A User's Guide, ed. Kiernan, J. A.
& Mason, I. G. London: Portland Press.
Kiernan,
J. A. 2002. Staining and counterstaining to show structural features.
Chapter 10 in Microscopy and Histology for Molecular Biologists. A
User's Guide, ed. Kiernan, J. A. & Mason, I. G. London:
Portland Press.
Kiernan,
J. A. 2002. Nomenclature and classification of dyes and other coloring
agents. Chapter 3 in Horobin, R. W. & Kiernan, J. A. (eds)
Conn's Biological Stains. A Handbook of Dyes and Fluorochromes for use
in Biology and Medicine. 10th ed. Oxford: BIOS. pp. 23-39.
Kiernan,
J. A. & Horobin, R. W. 2002. Miscellaneous inorganic and
organic substances used as biological stains. Chapter 27 in Horobin, R.
W. & Kiernan, J. A. (eds) Conn's Biological Stains. A Handbook
of Dyes and Fluorochromes for use in Biology and Medicine. 10th ed.
Oxford: BIOS. pp. 389-415.
Bova,
G.
S., Eltoum, I. A., Kiernan, J. A., Siegal, G. P., Frost, A. R., Best,
C. J. M., Gillespie, J. W. & Emmert-Buck, M. R. 2004. Optimal
molecular profiling of tissue and tissue components: defining the best
processing and microdissection methods for biomedical applications. Ch.
2 in Su, G. H. ed. Pancreatic Cancer. Methods and Protocols. (Methods
in Molecular Medicine, Vol. 103). Totowa, NJ: Humana Press. pp. 15-66.
(Also as an e-Book:
http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-780-7&txtSearchStartPos=1)
Carriel,
V., Campos, F., Aneiros-Fernandez, J. & Kiernan J. A. 2017.
Tissue fixation and processing for the histological identification of
lipids. Chapter 14 in: Pellicciari C, Biggiogera M, eds. Histochemistry
of Single Molecules: Methods and Protocols. (Methods in Molecular Biology, vol. 1560) New York: Springer Science+Business Media LLC. pp. 197-206. (ISBN 9781493967889)
Other
publications
These
include a few unrefereed abstracts of presentations at meetings and several other informal publications. The only Abstracts
listed are those containing information that was not also published in
peer-reviewed papers.
Kiernan, J. A.
1965. Some histochemical observations relating to the so-called ground
substance of the central nervous system. Journal of Anatomy 99, 926-928.
Kiernan,
J. A. 1965. Application of a fluorescent periodic acid-Schiff technique
to the central nervous system. Journal of Anatomy 99, 940.
Kiernan, J.
A. 1970. Two types of axonal regeneration in the neurohypophysis of the
rat. Journal of Anatomy 107, 187.
Kiernan,
J. A. 1971. Subcellular studies of axonal argyrophilia. Journal of
Anatomy 108, 623.
Kiernan,
J. A. 1996. Murray Llewellyn Barr (1908-1995). (Obituary) Journal of
Anatomy 188, 747-748.
Kiernan,
J. A. 1997. Making and using aqueous mounting media. Microscopy Today
97-10, 16-17.
Kiernan,
J. A. 1998. Double embedding in cellulose nitrate and paraffin wax, an
old and useful method that is often misunderstood. Microscopy Today
98-2, 12-14.
Kiernan,
J. A. 1999. Long-lasting aqueous mounts for light microscopy.
Microscopy Today 99-1, 3.
Kiernan,
J. A. 1999. Strategies for preventing detachment of sections from glass
slides. Microscopy Today 99-6, 22-24.
Kiernan,
J. A. 1999. Recycling gold. Microscopy Today 99-10, 23-25.
Kiernan,
J. A. 1999. Staining and microscopy: the need for old and new methods.
(Guest editorial) Biotechnic and Histochemistry 74, 275.
Kiernan,
J. A. 2000. Formaldehyde, formalin, paraformaldehyde and
glutaraldehyde: What they are and what they do. Microscopy Today 00-1,
8-12.
Kiernan,
J. A. 2000. Rit scarlet No. 5 as a stain for amyloid. (Letter,
commenting on paper in J. Histotechnol. 22: [1] 33-35, 1999) Journal of
Histotechnology 23, 87.
Kiernan,
J. A. 2000. Preventing lost tissues: a look at charged slides. What are
the exact differences between poly-L-lysine and aminoalkyloxysilanes?
Paraffin Press (Connecticut Society of Histotechnologists) Spring 2000,
3.
Kiernan,
J. A. 2000. Dehydrating delicate specimens for light microscopy.
Microscopy Today 00-7, 37.
Kiernan,
J. A. 2001. How to stain motor end plates. Microscopy Today 01-5: 29.
Kiernan,
J. A. 2001. How acids and chelating agents soften bones and teeth. Hard
times. Communiqué‚ of the Hard Tissue Committee,
National Society for Histotechnology 13 (2) July 2001. (mini-review
article)
Kiernan,
J. A., Pyle, R. H. & Silverman, J. S. 2001. N.S.H. Self
Assessment Examinations, Series 5: Immunohistochemistry, Enzyme
Histochemistry, Flow Cytometry, In-Situ Hybridization, Electron
Microscopy. 2nd ed. Bowie, MD, National Society for Histotechnology.
Kiernan,
J. A. (section editor) 2002- . Notes and Queries. A regular feature
comprising peer-reviewed answers to readers' questions. Biotechnic
& Histochemistry Vol. 76 onwards.
Kiernan,
J. A. 2005. Preservation and retrieval of antigens for
immunohistochemistry - methods and mechanisms. 1. Effects of
formaldehyde fixation. The Cutting Edge January 2005, pp. 5-9.
(National Society for Histotechnology, Newsletter for Region IX, Canada)
Kiernan,
J. A. 2005. Preservation and retrieval of antigens for
immunohistochemistry - methods and mechanisms. Part 2. Retrieving
masked antigens. The Cutting Edge April 2005, pp. 5-11. (National
Society for Histotechnology, Newsletter for Region IX, Canada)
Kiernan,
J. A. 2005. Nissl: the man, the stain and the substance. Microscopy
Today 13(6), 50.
Kiernan,
J. A. & Sims, S. M. 2006. Preface, in Hudson, A. J.
(2006) The Physiological Basis of Memory and Consciousness. Lewiston,
NY: Edwin Mellen Press.
Lyon,
H. O. & Kiernan, J. A. 2008. News from the Biological Stain
Commission. Biotechnic & Histochemistry 83: 201-203.
Lyon,
H. O. & Kiernan, J. A. 2008. News from the Biological Stain
Commission. Biotechnic & Histochemistry 83: 285-288.
Kiernan, J. A. 2008.
Nuclear Stains. Cold Spring Harbor Protocols 3:(7): doi:
10.1101/pdb.top50 (14 pages).
Kiernan, J. A. 2008.
Anionic counterstains. Cold Spring Harbor Protocols 3:(7): doi:
10.1101/pdb.top51 (2 pages).
Kiernan,
J. A. & Horobin, R. W. 2010. A special issue devoted to
hematoxylin, hematein and hemalum. Biotechnic &
Histochemistry 85:
5-6. (Editorial).
Kumar,
G. L. & Kiernan, J. A. 2010. Editorial. Special
stains and
H&E. Connection (Dako Inc. Scientific Magazine). Vol. 14, p. 14.
Kiernan,
J. A. 2010. Staining sections of the central nervous system. Connection
(Dako Inc. Scientific Magazine). Vol. 14, pp. 60-67.
Kiernan, J. A. 2010.
Carbohydrate histochemistry. Connection (Dako Inc. Scientific
Magazine). Vol. 14, pp. 68-78.
Kiernan,
J. A. 2010. On chemical reactions and staining mechanisms. Connection
(Dako Inc. Scientific Magazine). Vol. 14, pp. 127-136. [What is the
difference between argentaffin and argyrophilic reactiuons? (p.
127-128). How does pH influence the staining mechanism of Schiff's
reagent in biological tissue samples? (p. 129-131). What is
Giemsa’s
stain and how does it color blood cells, bacteria and chromosomes? (p.
132-136).]
Kumar,
G. L. & Kiernan, J. A. Preface, in Kumar, G. L. &
Kiernan, J.
A. (eds) 2010. Education Guide: Special Stains and H & E. 2nd
ed.
Carpinteria, CA: Dako North America, p. ix.
Kiernan,
J. A. 2013. Editorial. Journal of Histotechnology 36(1):
1-2. DOI:
10.1179/0147888513Z.00000000041 (Summary and brief discussion of papers
in the March 2013 issue of the journal)
Kiernan, J. A. 2014. Editorial. Journal of Histotechnology 37(2): 41-42. DOI: 10.1179/0147888514Z.00000000085 (Summary and brief discussion of papers
in the June 2014 issue of the journal)
Kiernan, J. A. 2016. Editorial: Innovations, applications and more innovations - a continuing tradition in histotechnical publication. Journal
of Histotechnology 39(2): 37-38. DOI:
10.1080/01478885.2016.1173969 (Comments on papers in the
June 2016 issue of the journal)
Kiernan,
J. A. 2017. Editorial. Fibers, bacteria and things that look like fungi
under the microscope. Journal of Histotechnology 40(2): 29-30.
Kiernan,
J. A. 2018. Book review: Fluorescence Microscopy in Life Sciences, by
Juan Carlos Stockert and Alfonso Blázquez-Castro. Bentham Science
Publishers, Sharjah, UAE, 2017. Journal of Histotechnology 41(4):
195-196. DOI:10.1080/014778885.2018.1514715 https://www.tandfonline.com/eprint/Z4sCX7KkdhFBh37UfsrK/full
Dapson,
R. W., Horobin, R. W., Kiernan, J. A. 2019. Glossary of staining
methods, reagents, immunostaining, terminology and eponyms. Version 1.2. Biological
Stain Commission, Inc. https://biologicalstaincommission.org/bscglossary.html
