J.B. Stothers NMR Facility
Department of Chemistry
Western University
London, Ontario, Canada
NMR Service - Liquid/Solution Samples
About Liquid-State NMR Spectroscopy

The vast majority of NMR samples are studied in the liquid-state where solid samples are dissolved in the appropriate deuterated solvent. In order to study a sample by liquid-state NMR spectroscopy:
  • Enough sample must be provided to make a 0.6 mL solution of at least 1mM. Usually more sample is required and the solution concentration required will depend on the desired NMR nucleus of interest and experiment. In most cases, solutions of at least 10mM are preferred. If your sample is not appreciably soluble in any known solvent, you may consider using solid-state NMR spectroscopy instead.
  • The sample should be diamagnetic. Paramagnetic samples can be studied by NMR, but the NMR is much more complicated and may not give rise to any useable data.
Services Provided

Depending on your experience using NMR spectroscopy as a means of structural characterization, the NMR facility can perform a variety of functions. For experienced users, staff generally just acquire the NMR data and provide the raw NMR data. For novice users, the NMR facility can prepare the NMR sample, acquire the NMR data, process and completely analyze the NMR spectra. For all external service work, a report summarizing the NMR experiments performed and the experimental conditions utilized is included. Experiments are typically completed within 48 hours of sample receipt. If complete analysis is required, at least 1 week is necessary.

The NMR facility provides a base service for all samples, which includes:
  • NMR tube
  • NMR solvent (deuterated solvent)
  • preparation of solution for NMR
  • acquisition of a 1D 1H NMR spectrum at 25 oC
  • e-mailing of raw NMR data (FID)
  • service summary report outlining the NMR experiments performed, NMR conditions and charges (external samples only)
  • sample disposal
For an additional charge above that of the base service, the NMR facility can perform the following NMR experiments:
  • Standard direct-detect 1D NMR experiments on C-13, F-19 and P-31.
  • Odd-nuclei direct-detect 1D NMR experiments on nuclei such as H-2, B-11, Se-77. Many other nuclei can be studied as well. Please enquire.
  • Indirect-detect heteronuclear 2D NMR experiments on nuclei such as N-15, Si-29. Many other nuclei can be studied as well. Please enquire.
  • Homonuclear 2D 1H-1H NMR experiments such as COSY, TOCSY, NOESY/ROESY.
  • Any of the above experiments can also be performed above or below room-temperature.
For an additional charge above that of the base service, the NMR facility will perform or help with the following tasks:
  • Processing of the the NMR data into an NMR spectrum.
  • Analysis of the NMR spectra, including integration, peak picking, and peak assignments where applicable.
  • Interpretation of 1D and 2D NMR spectra.
External Service Rates

The rates for NMR service for non-Western clients can be found in the table below. Unless otherwise specified, the prices are per sample, are in Canadian dollars, and are effective until April 30, 2014.  If desired, one can also download the Liquids NMR Service Rates in a PDF file.

Rates for Liquids NMR Service
Experiment Academia / Not for Profit Industry Comments
Base Service $30 $50 Base service includes:
  • NMR solventa
  • NMR tube
  • preparation of NMR solution
  • acquisition of 1H NMR spectrum at 25 °C
  • e-mailing of raw 1H NMR data (the 1H NMR FID)
  • service summary report (external samples only)
  • sample disposal
+Additional Charges (for any additional experiments, processing or analysis above the base service)
Processing and Analysis
Basic analysis $15 $25 Includes electronic PDF plots with peak picking and integration (if applicable).
Interpretation $50/hour $100/hour Charge for NMR facility staff to provide interpretation of the NMR spectra, spectral assignments and a written report explaining the interpretation.
Variable-Temperature
> 25 °C $15 $25 Charge per temperature point.  Maximum temperature is +120 °C.
< 25 °C $25 $35 Charge per temperature point.  Minimum temperature is −80 °C.
1D NMR Experimentsb
1H $10 $15 For each additional 1H spectrum acquired above or below room temperature.
13C $20 $30 Charge per experiment per temperature in addition to the base service price.
19F $10 $15 Charge per experiment per temperature in addition to the base service price.
31P $15 $20 Charge per experiment per temperature in addition to the base service price.
{1H,1H} Selective $15 $20 Includes 1D TOCSY, NOESY or ROESY. Charge per irradiation point per temperature in addition to the base service price.
Homonuclear 2D NMR Experimentsb
{1H,1H} gCOSY $15 $25 Charge per experiment per temperature in addition to the base service price.
{1H,1H} TOCSY $20 $35 Charge per experiment per temperature in addition to the base service price.
{1H,1H} NOESY $50 $100 Same price for a ROESY. Charge per experiment per temperature in addition to the base service price.
Heteronuclear 2D NMR Experimentsb
{1H,1C} gHSQC $20 $35 Charge per experiment per temperature in addition to the base service price.
{1H,1C} gHMBC $30 $55 Charge per experiment per temperature in addition to the base service price.
aSolvents are between 99.5% to 99.9%-d and are stored in individual sealed ampoules. The price quoted applies only to acetone-d6, benzene-d6, CDCl3, DMSO-d6, and D2O. Prices will be provided upon request for higher deuteration or other solvents such as acetonitrile-d3, CD2Cl2, methanol-d4, THF-d8, etc..
bNMR experiments can be performed on nuclei other than those listed in the table; prices will be provided upon request.
Sample Submission Procedure for Western Personnel

The following procedure should be used when submitting a sample for service work:
  1. Contact NMR facility staff via e-mail at chemnmr@uwo.ca or phone at 519-661-2111 x. 82905 to discuss what information you are looking to obtain by using NMR, what solvent should be used, the nature of your sample and what services are required.
  2. Fill out and print the Liquids NMR Service Request - Western Personnel form. Note that the form is a fillable PDF form that can be filled out electronically.
  3. If you are preparing your own NMR sample, bring the NMR sample and the completed sample submission form to the NMR facility. If you are not preparing your own NMR sample, bring at least 20mg of sample in a screw-top vial and the completed sample submission form to the NMR facility.
  4. Requested data will be sent via e-mail generally within 48 hours of sample receipt.
Sample Submission Procedure for External Clients (Non-Western Personnel)

The following procedure should be used when submitting a sample for service work:
  1. Contact NMR facility staff via e-mail at chemnmr@uwo.ca or phone at 519-661-2111 x. 82905 to discuss what information you are looking to obtain by using NMR, what solvent should be used, the nature of your sample and what services are required.
  2. Fill out and print the Liquids NMR Service Request - External Clients. Note that the form is a fillable PDF form that can be filled out electronically.
  3. If you are preparing your own NMR sample, bring the NMR sample and the completed sample submission form to the NMR facility. If you are not preparing your own NMR sample, bring at least 20mg of sample in a screw-top vial and the completed sample submission form to the NMR facility. If you are off-campus, the sample can be mailed, along with the completed sample submission form to:
    4. Mathew Willans
    Western University
    1151 Richmond St. N.
    Department of Chemistry
    Dock 003, Room 11
    London, ON
    N6A 5B7
  4. Requested data will be sent via e-mail generally within 48 hours of sample receipt. Depending on the analysis required, the sample report will generally be sent via e-mail within 7 days after all NMR spectra have been acquired.