Files containing directly available information (text, tables,
will be displayed if
you click on the highlighted link
Files with names of the form filename.zip are archives made with the PKZIP program. When you click on a link to an archive (.ZIP) you will be invited to download the file. Each archive contains several compressed files. To use a .ZIP archive you must download it and then decompress it (with PKUNZIP, WINZIP, WINRAR or a similar un-archiving program). PDF (portable document format) files require the Adobe Acrobat Reader. If you don't have this free program, click here to download it.
Histochemistry1. Utilities for chemical calculations:
2. Practical instructions with explanations
3. Links to other web sites
Click here for more information about the histochemistry and histotechnology files and links for downloading.
NeuroanatomyThe University of Western Ontario employs me to teach this subject.
Click here for more information about the neuroanatomy files and links for downloading.
Other GoodiesThese are utilities for people who know how to use DOS. Most were written and compiled with ASIC (Version 5). ASIC is a dialect of BASIC written by David Visti. It generates very small .COM or .EXE files that run quickly and efficiently.
The files include:
For these files to work under recent Windows operating systems (Windows 7 or 10; 32-bit or 64-bit), it is necessary to install DOSBox, a free (public domain) emulation program. Click here to get the latest version of DOSBox.
I will be happy to provide the ASIC source code to anyone interested in trying to bring one or more of these utilities up to date. Contact email: kiernan[AT]uwo.ca
Click here for more information about ASIC, including programming and library files for downloading.
Histochemistry and histotechnology files
|Utilities for chemical calculations.
These include three programs that calculate data about entered
chemical formulae. For example, if you enter:
MW NaHCO3 the
formula and molecular weight
of sodium bicarbonate will appear on the screen as NaHCO3 84.00687. This result can be sent to a
comma-delimited database file if desired.
With the solutions utility you could type SOLUTION H2NCONH2 6.5M and obtain the concentration of 6.5M urea in various units, including 39.04 grams per 100 ml. You can also use SOLUTION to obtain molarity from grams per litre or percent w/v.
For a peptide sequence, PEP gly-lys-trp-arg tells you that this tetrapeptide has molecular weight 545.67 and that its pI is approximately 8.7.
You will need to install the DOSBox emulation utility (free download) to run these utilities on a computer with Windows 7.
|Download chemical utilities. DOS version (66513 bytes)|
|FAQ: Staining, histochemistry and histotechnology. An indexed collection of frequently asked questions on 84 topics, grouped in 6 major categories. Some topics include more than one question and answer. Many are documented with references to books and other publications. Several people have contributed answers, many of which have appeared on the Histonet listserver. This FAQ is a single quite large file (220 KB) so it may take a minute or two to load by way of a telephone connection. Once loaded, movement among its links is instantaneous. This FAQ will be updated from time to time.||Consult the FAQ|
|HistoTech. This DOS
program generates instructions for making solutions or carrying out
staining methods. When the program is started by entering the
the user is presented with a
choice of menus (alphabetical or by type) and invited to select
an item such as Bouin's
fluid or Luxol fast blue for myelin.
The program makes a plain text file containing instructions for making
the fixative or doing the technique (including making up all the
solutions used). A text file
can be edited or printed using any word processing program or text
The database is not complete, but worksheets can be prepared for scores of solutions and techniques, including a few that aren't in all the textbooks. Unfortunately this program refuses to run on computers with 700 MHz or faster processors (and DOSBox does not solve the problem).
|Download HISTOTEC.ZIP (93625 bytes)|
|Keeping sections on slides. A review of the approaches to this old problem: positively charged slides (APES, polylysine); adhesives (especially chrome gelatin); and other tricks too.||Read about adhesives for keeping sections on slides.|
|Coating slides with nitrocellulose (= celloidin or collodion). Detailed instructions for encasing slides in nitrocellulose film to prevent removal of sections by harsh liquids such as alkalis and hot acids. Includes references.||Read how to treat slides with nitrocellulose.|
|Making your own aqueous mounting media. Instructions for making aqueous mountants. Why buy, when it's cheap and easy to make your own? Originally published in Microscopy Today, and reproduced with permission.||Recipes and instructions for 4 aqueous mountants.|
|Formaldehyde, paraformaldehyde and glutaraldehyde. What they are and what they do. A short review of how aldehyde fixatives work, with chemical schematics and references. Originally published in Microscopy Today, and reproduced with permission.||Read about formaldehyde and glutaraldehyde.|
|How to stain motor end plates. A brief overview, with references, of combined techniques for acetylcholinesterase activity and the terminal branches of motor axons in skeletal muscle. Originally published in Microscopy Today, and reproduced with permission.||Read about staining neuromuscular innervation.|
|Formaldehyde fixation and immunohistochemistry. An article that appeared in an NSH regional newsletter in 2005, explaining the actions of formaldehyde and why this fixative often impairs subsequent application of immunohistochemical methods. (PDF file)||Read about actions of formaldehyde on antigens|
|Antigen retrieval. Another article that appeared in an NSH regional newsletter in 2005, discussing procedures for unmasking antigens in sections of formaldehyde-fixed tissue. The possible mechanisms of several antigen retrieval procedures are discussed. (PDF file)||Read about antigen retrieval|
|Click here for some links to histological, histochemical and histotechnical web sites.|
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|Anatomical foundations of neuroscience. This is an HTML text file with links to more than 60 images. It is based on handout notes (about 115 pages when printed) for a graduate half-course (Anatomy 535b) given at the University of Western Ontario. The notes explain the cells of the nervous system, developmental and comparative neuroanatomy and regional and functional anatomy of the nervous systems of man, the rat and some other animals.||This file, with its links to pictures and tables of data, is designed to be read on screen.|
|Neuro-Slides. These are sets of images used in lectures to medical, allied health sciences and graduate students. Each set is a stand-alone .EXE file that displays the images on screen or with an LCD projector. (Needs Windows 9x, NT, 2000 or XP.)||Click here for a list of slide-shows with descriptions and instructions for downloading.|
|Dissection of the human brain. Instructions for dissecting the human brain may be read on-screen, or you may prefer to download the zipped text file. There are references to photographic illustrations in Barr's The Human Nervous System: An Anatomical Viewpoint, 8th edn by J. A. Kiernan (Philadelphia: Lippincott, Williams & Wilkins, 2005 ISBN 0-397-58431-8 ). These photos show many stages of the dissection and also stained sections of the cerebrum, brain stem and spinal cord.||Dissection of the human brain (On screen)|
|Neuro Quiz. A collection
of about 300 questions, grouped by subject. Answer by typing a word or
phrase. The correct answer is then shown and explained.
This program works well in a DOSBox window in Windows 7.
|All the files from the NEUROQUIZ.ZIP archive must reside in the same directory. The command BQ starts the program. For more information read the text file BQ.DOC|
|Neuro-MCQ. About 140
multiple choice questions in Neuroanatomy. They can be chosen in groups
or at random from the whole collection.
This program works well in a DOSBox window in Windows 7.
|A single file, NMCQ.EXE, is all that is needed to run this program.|
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Last updated: March 2017